Two-Dimensional Algal Collection and Assembly by Combining AC-Dielectrophoresis with Fluorescence Detection for Contaminant-Induced Oxidative Stress SensingReportar como inadecuado




Two-Dimensional Algal Collection and Assembly by Combining AC-Dielectrophoresis with Fluorescence Detection for Contaminant-Induced Oxidative Stress Sensing - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Environmental Biogeochemistry and Ecotoxicology, Institute F.-A. Forel, Earth and Environmental Science, Faculty of Sciences, University of Geneva, 10 route de Suisse, Versoix CH-1290, Switzerland

2

Department of Chemical and Biomolecular Engineering, North Carolina State University, Raleigh, NC 27695, USA





*

Author to whom correspondence should be addressed.



Academic Editor: Christophe A. Marquette

Abstract An alternative current AC dielectrophoretic lab-on-chip setup was evaluated as a rapid tool of capture and assembly of microalga Chlamydomonas reinhardtii in two-dimensional 2D close-packed arrays. An electric field of 100 V·cm−1, 100 Hz applied for 30 min was found optimal to collect and assemble the algae into single-layer structures of closely packed cells without inducing cellular oxidative stress. Combined with oxidative stress specific staining and fluorescence microscopy detection, the capability of using the 2D whole-cell assembly on-chip to follow the reactive oxygen species ROS production and oxidative stress during short-term exposure to several environmental contaminants, including mercury, methylmercury, copper, copper oxide nanoparticles CuO-NPs, and diuron was explored. The results showed significant increase of the cellular ROS when C. reinhardtii was exposed to high concentrations of methylmercury, CuO-NPs, and 10−5 M Cu. Overall, this study demonstrates the potential of combining AC-dielectrophoretically assembled two-dimensional algal structures with cell metabolic analysis using fluorescence staining, as a rapid analytical tool for probing the effect of contaminants in highly impacted environment. View Full-Text

Keywords: whole cell assembly; dielectrophoresis; fluorescence; contamination; reactive oxygen species; Chlamydomonas reinhardtii whole cell assembly; dielectrophoresis; fluorescence; contamination; reactive oxygen species; Chlamydomonas reinhardtii





Autor: Coralie Siebman 1, Orlin D. Velev 2 and Vera I. Slaveykova 1,*

Fuente: http://mdpi.com/



DESCARGAR PDF




Documentos relacionados