Characterization of Lactate Sensors Based on Lactate Oxidase and Palladium Benzoporphyrin Immobilized in HydrogelsReportar como inadecuado




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1

Department of Biomedical Engineering, 5045 Emerging Technologies Building, 3120 TAMU, Texas A&M University, College Station, TX 77843, USA

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PROFUSA, Inc., 345 Allerton Avenue, South San Francisco, CA 94080, USA

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Department of Materials Science and Engineering, 3003 TAMU, Texas A&M University, College Station, TX 77843, USA





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Author to whom correspondence should be addressed.



Academic Editor: A. Sheila Holmes-Smith

Abstract An optical biosensor for lactate detection is described. By encapsulating enzyme-phosphor sensing molecules within permeable hydrogel materials, lactate-sensitive emission lifetimes were achieved. The relative amount of monomer was varied to compare three homo- and co-polymer materials: poly2-hydroxyethyl methacrylate pHEMA and two copolymers of pHEMA and polyacrylamide pAam. Diffusion analysis demonstrated the ability to control lactate transport by varying the hydrogel composition, while having a minimal effect on oxygen diffusion. Sensors displayed the desired dose-variable response to lactate challenges, highlighting the tunable, diffusion-controlled nature of the sensing platform. Short-term repeated exposure tests revealed enhanced stability for sensors comprising hydrogels with acrylamide additives; after an initial -break-in- period, signal retention was 100% for 15 repeated cycles. Finally, because this study describes the modification of a previously developed glucose sensor for lactate analysis, it demonstrates the potential for mix-and-match enzyme-phosphor-hydrogel sensing for use in future multi-analyte sensors. View Full-Text

Keywords: luminescence; lactate; enzyme; biosensor; hydrogel luminescence; lactate; enzyme; biosensor; hydrogel





Autor: Liam P. Andrus 1, Rachel Unruh 1, Natalie A. Wisniewski 2 and Michael J. McShane 1,3,*

Fuente: http://mdpi.com/



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