Design and Characterization of a Sensorized Microfluidic Cell-Culture System with Electro-Thermal Micro-Pumps and Sensors for Cell Adhesion, Oxygen, and pH on a Glass ChipReportar como inadecuado




Design and Characterization of a Sensorized Microfluidic Cell-Culture System with Electro-Thermal Micro-Pumps and Sensors for Cell Adhesion, Oxygen, and pH on a Glass Chip - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Chair for Biophysics, Department of Biology, University of Rostock, Gertrudenstr. 11a, 18057 Rostock, Germany

2

Leibniz Institute for Farm Animal Biology, Institute of Muscle Biology and Growth, Wilhelm-Stahl-Allee 2, 18196 Dummerstorf, Germany

3

DOT GmbH, Charles-Darwin-Ring 1A, 18059 Rostock, Germany





*

Author to whom correspondence should be addressed.



Academic Editor: Christophe A. Marquette

Abstract We combined a multi-sensor glass-chip with a microfluidic channel grid for the characterization of cellular behavior. The grid was imprinted in poly-dimethyl-siloxane. Mouse-embryonal-fetal calvaria fibroblasts MC3T3-E1 were used as a model system. Thin-film platinum Pt sensors for respiration amperometric oxygen electrode, acidification potentiometric pH electrodes and cell adhesion interdigitated-electrodes structures, IDES allowed us to monitor cell-physiological parameters as well as the cell-spreading behavior. Two on-chip electro-thermal micro-pumps ETμPs permitted the induction of medium flow in the system, e.g., for medium mixing and drug delivery. The glass-wafer technology ensured the microscopic observability of the on-chip cell culture. Connecting Pt structures were passivated by a 1.2 μm layer of silicon nitride Si3N4. Thin Si3N4 layers 20 nm or 60 nm were used as the sensitive material of the pH electrodes. These electrodes showed a linear behavior in the pH range from 4 to 9, with a sensitivity of up to 39 mV per pH step. The oxygen sensors were circular Pt electrodes with a sensor area of 78.5 μm2. Their sensitivity was 100 pA per 1% oxygen increase in the range from 0% to 21% oxygen air saturated. Two different IDES geometries with 30- and 50-μm finger spacings showed comparable sensitivities in detecting the proliferation rate of MC3T3 cells. These cells were cultured for 11 days in vitro to test the biocompatibility, microfluidics and electric sensors of our system under standard laboratory conditions. View Full-Text

Keywords: lab-on chip; bone cells; thin film platinum sensors; acidification; respiration lab-on chip; bone cells; thin film platinum sensors; acidification; respiration





Autor: Sebastian M. Bonk 1, Marco Stubbe 1, Sebastian M. Buehler 2, Carsten Tautorat 1, Werner Baumann 1, Ernst-Dieter Klinkenberg 3 and Jan Gimsa 1,*

Fuente: http://mdpi.com/



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