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1

Centre for Microscopy and Imaging, National University Ireland, University Road, Galway, Ireland

2

School of Physics, National University Ireland, University Road, Galway, Ireland

3

Bio-Optical Imaging Group, Singapore Bioimaging Consortium, Agency for Science Technology and Research A*STAR, 11 Biopolis Way, #02-02 Helios 138667, Singapore



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Yuliya Semenova

Abstract In this paper we describe a method for the determination of protein concentration using Surface Enhanced Raman Resonance Scattering SERRS immunoassays. We use two different Raman active linkers, 4-aminothiophenol and 6-mercaptopurine, to bind to a high sensitivity SERS substrate and investigate the influence of varying concentrations of p53 and EGFR on the Raman spectra. Perturbations in the spectra are due to the influence of protein–antibody binding on Raman linker molecules and are attributed to small changes in localised mechanical stress, which are enhanced by SERRS. These influences are greatest for peaks due to the C-S functional group and the Full Width Half Maximum FWHM was found to be inversely proportional to protein concentration. View Full-Text

Keywords: Raman spectroscopy; protein sensing; surface-enhanced Raman scattering; biomarker Raman spectroscopy; protein sensing; surface-enhanced Raman scattering; biomarker





Autor: Peter Owens 1,†,* , Nigel Phillipson 2,†, Jayakumar Perumal 3, Gerard M. O’Connor 2 and Malini Olivo 2,3

Fuente: http://mdpi.com/



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