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Laboratoire de Génie Enzymatique, Membranes Biomimétiques et Assemblages Supramoléculaires—Institut de Chimie et Biochimie Moléculaires et Supramoléculaires—Université Claude Bernard Lyon 1, Villeurbanne 69100, France





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Academic Editor: Roberto Pilloton

Abstract In the present report, we are making the proof of concept of cell small populations from 1 to 100 cells spotting, culture and secretion detection on a gold surface. In order to keep the cells in a hydrated environment during the robotized micropipetting and to address different cell lines on a single chip, a biocompatible alginate polymer was used. This approach enables the encapsulation of the cell in a very small volume 30 nL, directly on the substrate and permits a precise control of the number of cells in each alginate bead. After 24 h of culture, the adherent cells are ready for surface plasmon resonance imaging SPRi experimentation. To enable the detection of secreted proteins, various antibodies are immobilized in an organized manner on a SPRi sensor and permitted the multiplex detection of different proteins secreted by the different cultured cell lines. Evidence of the real-time detection will be presented for Prostate Specific Antigen PSA and β-2-microglobulin B2M secreted by prostate cancer cells following induction by dihydrotestosterone DHT. Different kinetics for the two secreted proteins were then demonstrated and precisely determined using the chip. View Full-Text

Keywords: biosensor; cell microarray; cell encapsulation; SPRi; real-time biosensor; cell microarray; cell encapsulation; SPRi; real-time





Autor: Ophélie I. Berthuy, Loïc J. Blum and Christophe A. Marquette *

Fuente: http://mdpi.com/



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