Mineralization and Characterization of Composite Lyophilized Gelatin Sponges Intended for Early Bone RegenerationReportar como inadecuado


Mineralization and Characterization of Composite Lyophilized Gelatin Sponges Intended for Early Bone Regeneration


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1

Department of Biomedical Engineering, The University of Memphis and Joint University of Memphis-UTHSC-Memphis Biomedical Engineering Program, 119D Engineering Technology, Memphis, TN 38152, USA

2

Department of Biomedical Sciences, Heritage College of Osteopathic Medicine, Ohio University, Grosvenor Hall, Athens, OH 45701, USA

3

Department of Biomedical Engineering, Parks College of Engineering, Aviation, and Technology, Saint Louis University, 3507 Lindell Blvd., St. Louis, MO 63103, USA





*

Author to whom correspondence should be addressed.



Abstract The application of freeze-dried gelatin sponges as alternative bone grafting substitutes has many advantages, including the ability to swell, high porosity, tailorable degradation, and versatility to incorporate multiple components such as growth factors and nanofillers. The purpose of this study was to mineralize M and further characterize 1-Ethyl-3-3-dimethylaminopropylcarbodiimide hydrochloride EDC cross-linked gelatin sponges enhanced with preparations rich in growth factors, hydroxyapatite, and chitin whiskers PHCE. Sponges were characterized for their swelling and in vitro mineralization potential, surface characteristics, protein release, mechanical properties, and MG-63 cell attachment and infiltration. All sponges swelled up to 50% of their original volume upon hydration. Scanning electron microscopy showed sparse mineral deposition for gelatin-M scaffolds while PHCE-M scaffolds exhibited more uniform mineral nucleation. Over 21 days, PHCE-M scaffolds cumulatively released significantly more 30% of its initial protein content than all other scaffolds. PHCE-M scaffolds reported lower modulus values 1.3–1.6 MPa when compared to gelatin control scaffolds 1.6–3.2 MPa. Increased cell attachment and infiltration was noticed on PHCE and PHCE-M scaffolds. The results of the study demonstrate the enhanced performance of PHCE and PHCE-M scaffolds to serve as bone healing scaffolds. Their potential to release incorporated factors, comparable composition-mechanical properties to tissues developed in the early stages of bone healing, and enhanced initial cellular response make them suitable for further studies evaluating more complex cellular interactions. View Full-Text

Keywords: bone tissue engineering; platelet-rich plasma; hydroxyapatite; chitin; gelatin; simulated body fluid; bone graft substitute bone tissue engineering; platelet-rich plasma; hydroxyapatite; chitin; gelatin; simulated body fluid; bone graft substitute





Autor: Isaac Rodriguez 1, Gunjan Saxena 2, Scott Sell 3 and Gary Bowlin 1,*

Fuente: http://mdpi.com/



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