Characterization and Application of a Disposable Rotating Bed Bioreactor for Mesenchymal Stem Cell ExpansionReportar como inadecuado


Characterization and Application of a Disposable Rotating Bed Bioreactor for Mesenchymal Stem Cell Expansion


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1

Department for Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria

2

Institute for Technical Chemistry, Gottfried Wilhelm Leibniz University Hanover, Callinstrasse 5, 30167 Hanover, Germany



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Christoph Herwig

Abstract Recruitment of mesenchymal stromal cells MSC into the field of tissue engineering is a promising development since these cells can be expanded vivo to clinically relevant numbers and, after expansion, retain their ability to differentiate into various cell lineages. Safety requirements and the necessity to obtain high cell numbers without frequent subcultivation of cells raised the question of the possibility of expanding MSC in one-way single-use disposable bioreactors. In this study, umbilical cord-derived MSC UC-MSC were expanded in a disposable Z 2000 H bioreactor under dynamic conditions. Z was characterized regarding residence time and mixing in order to evaluate the optimal bioreactor settings, enabling optimal mass transfer in the absence of shear stress, allowing an reproducible expansion of MSC, while maintaining their stemness properties. Culture of the UC-MSC in disposable Z 2000 H bioreactor resulted in a reproducible 8-fold increase of cell numbers after 5 days. Cells were shown to maintain specific MSC surface marker expression as well as trilineage differentiation potential and lack stress-induced premature senescence. View Full-Text

Keywords: umbilical cord stem cells; MSC expansion; dynamic culture; bioreactor umbilical cord stem cells; MSC expansion; dynamic culture; bioreactor





Autor: Anne Neumann 1,2,†, Antonina Lavrentieva 2,†, Alexandra Heilkenbrinker 2, Maren Loenne 2 and Cornelia Kasper 1,*

Fuente: http://mdpi.com/



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