Antioxidant Activity of Oat Proteins Derived Peptides in Stressed Hepatic HepG2 CellsReportar como inadecuado


Antioxidant Activity of Oat Proteins Derived Peptides in Stressed Hepatic HepG2 Cells


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1

Food Science and Nutrition, Department of Chemistry, Carleton University, Ottawa, ON K1S 5B6, Canada

2

Department of Biology and Institute of Biochemistry, Carleton University, Ottawa, ON K1S 5B6, Canada





*

Author to whom correspondence should be addressed.



Academic Editor: David Burritt

Abstract The purpose of this study was to determine, for the first time, antioxidant activities of seven peptides P1–P7 derived from hydrolysis of oat proteins in a cellular model. In the oxygen radical absorbance capacity ORAC assay, it was found that P2 had the highest radical scavenging activity 0.67 ± 0.02 µM Trolox equivalent TE-µM peptide followed by P5, P3, P6, P4, P1, and P7 whose activities were between 0.14–0.61 µM TE-µM. In the hepatic HepG2 cells, none of the peptides was cytotoxic at 20–300 µM. In addition to having the highest ORAC value, P2 was also the most protective 29% increase in cell viability against 2,2′-azobis2-methylpropionamidine dihydrochloride -induced oxidative stress. P1, P6, and P7 protected at a lesser extent, with an 8%–21% increase viability of cells. The protection of cells was attributed to several factors including reduced production of intracellular reactive oxygen species, increased cellular glutathione, and increased activities of three main endogenous antioxidant enzymes. View Full-Text

Keywords: oat peptides; cytoprotection; antioxidant enzymes; HepG2 cells oat peptides; cytoprotection; antioxidant enzymes; HepG2 cells





Autor: Yichen Du 1, Ramak Esfandi 1, William G. Willmore 2 and Apollinaire Tsopmo 1,*

Fuente: http://mdpi.com/



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