Acute Pre-Post-Treatment with 8th Day SOD-Like Supreme a Free Radical Scavenging Health Product Protects against Oxidant-Induced Injury in Cultured Cardiomyocytes and Hepatocytes In Vitro as Well as in Mouse Myocardium and Liver IReportar como inadecuado


Acute Pre-Post-Treatment with 8th Day SOD-Like Supreme a Free Radical Scavenging Health Product Protects against Oxidant-Induced Injury in Cultured Cardiomyocytes and Hepatocytes In Vitro as Well as in Mouse Myocardium and Liver I


Acute Pre-Post-Treatment with 8th Day SOD-Like Supreme a Free Radical Scavenging Health Product Protects against Oxidant-Induced Injury in Cultured Cardiomyocytes and Hepatocytes In Vitro as Well as in Mouse Myocardium and Liver I - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

1

Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China

2

Health Science Group Holdings Limited, Hong Kong SAR, China





*

Author to whom correspondence should be addressed.



Academic Editors: Gloria E. O. Borgstahl and Rebecca Oberley-Deegan

Abstract 8th Day superoxide dismutase SOD-Like Supreme SOD-Like Supreme, a free radical scavenging health product is an antioxidant-enriched fermentation preparation with free radical scavenging properties. In the present study, the cellular-tissue protective actions of SOD-Like Supreme against menadione toxicity in cultured H9c2 cardiomyocytes and in AML12 hepatocytes as well as oxidant-induced injury in the mouse myocardium and liver were investigated. SOD-Like Supreme was found to possess potent free radical scavenging activity in vitro as assessed by an oxygen radical absorbance capacity assay. Incubation with SOD-Like Supreme 0.5–3% v-v was shown to protect against menadione-induced toxicity in H9c2 and AML12 cells, as evidenced by increases in cell viability. The ability of SOD-Like Supreme to protect against menadione cytotoxicity was associated with an elevation in the cellular reduced glutathione GSH-oxidized glutathione GSSG ratio in menadione-challenged cells. Consistent with the cell-based studies, pre-post-treatment with SOD-Like Supreme 0.69 and 2.06 mL-kg, three intermittent doses per day for two consecutive days was found to protect against isoproterenol-induced myocardial injury and carbon tetrachloride hepatotoxicity in mice. The cardio-hepatoprotection afforded by SOD-Like Supreme was also paralleled by increases in myocardial-hepatic mitochondrial GSH-GSSG ratios in the SOD-Like Supreme-treated-oxidant-challenged mice. In conclusion, incubation-treatment with SOD-Like Supreme was found to protect against oxidant-induced injury in vitro and in vivo, presumably by virtue of its free radical scavenging activity. View Full-Text

Keywords: antioxidant; free radical scavenger; oxidative stress; glutathione; myocardium; liver antioxidant; free radical scavenger; oxidative stress; glutathione; myocardium; liver





Autor: Pou Kuan Leong 1, Jihang Chen 1, Wing Man Chan 1, Hoi Yan Leung 1, Lincoln Chan 2 and Kam Ming Ko 1,*

Fuente: http://mdpi.com/



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