Vol 13: Up-regulation of miR-224 promotes cancer cell proliferation and invasion and predicts relapse of colorectal cancer.Reportar como inadecuado



 Vol 13: Up-regulation of miR-224 promotes cancer cell proliferation and invasion and predicts relapse of colorectal cancer.


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This article is from Cancer Cell International, volume 13.AbstractBackground: MicroRNAs miRNAs are small, non-coding RNAs that can function as oncogenes or tumor suppressors in human cancer. Abnormally expressed miR-224 was found to play a fundamental role in several types of cancer. The aim of this study was to investigate the prognostic and biological values of miR-224 in colorectal cancer CRC. Methods: Quantitative RT-PCR qRT-PCR was used to evaluate expression levels of miR-224. The postoperative survival rate was analyzed with Kaplan–Meier method. The roles of miR-224 in cell proliferation, migration and invasion were analyzed with pre-miR-224 transfected cells. In addition, the regulation of SMAD4 by miR-224 was evaluated by qRT-PCR, Western blotting and luciferase reporter assays. Results: In the present study, we demonstrated that miR-224 was significantly up-regulated in CRC tissue samples and associated with disease relapse and a relative poorer disease-free survival rate. Moreover, ectopic expression of miR-224 potently promoted tumor cell proliferation, migration and invasion in vitro. Furthermore, the over-expression of miR-224 in CRC cell lines decreased SMAD4 expression at the translational level and decreased SMAD4-driven luciferase-reporter activity. Conclusions: Our data suggest that miR-224 could play an oncogenic role in the cellular processes of CRC and represent a novel biomarker for tumor relapse of CRC patients.



Autor: Zhang, Guang-jun; Zhou, He; Xiao, Hua-xu; Li, Yu; Zhou, Tong

Fuente: https://archive.org/



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