Vol 9: Uptake and Transfer of a Bt Toxin by a Lepidoptera to Its Eggs and Effects on Its Offspring.Reportar como inadecuado



 Vol 9: Uptake and Transfer of a Bt Toxin by a Lepidoptera to Its Eggs and Effects on Its Offspring.


Vol 9: Uptake and Transfer of a Bt Toxin by a Lepidoptera to Its Eggs and Effects on Its Offspring. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Descargar gratis o leer online en formato PDF el libro: Vol 9: Uptake and Transfer of a Bt Toxin by a Lepidoptera to Its Eggs and Effects on Its Offspring.
This article is from PLoS ONE, volume 9.AbstractResearch on non-target effects of transgenic crop plants has focused primarily on bitrophic, tritrophic and indirect effects of entomotoxins from Bacillus thuringiensis, but little work has considered intergenerational transfer of Cry proteins. This work reports a lepidopteran Chlosyne lacinia taking up a Bt entomotoxin when exposed to sublethal or low concentrations, transferring the entomotoxin to eggs, and having adverse effects on the first filial generation F1 offspring. Two bioassays were conducted using a sublethal concentration of toxin 100.0 ng-µl Cry1Ac for adults and a concentration equal to the LC10 2.0 ng-µl Cry1Ac for larvae. Cry1Ac is the most common entomotoxin expressed in Bt cotton in Brazil. In the adult diet bioassay there was no adverse effect on the parental generation P0 adults, but the F1 larvae had higher mortality and longer development time compared to F1 larvae of parents that did not ingest Cry1Ac. For the 3rd instar larvae, there was no measurable effect on the P0 larvae, pupae and adults, but the F1 larvae had higher mortality and longer development time. Using chemiluminescent Western Blot, Cry1Ac was detected in F1 eggs laid by P0 butterflies from both bioassays. Our study indicates that, at least for this species and these experimental conditions, a ∼65 kDa insecticidal protein can be taken up and transferred to descendants where it can increase mortality and development time.



Autor: Paula, Debora Pires; Andow, David A.; Timbo, Renata Velozo; Sujii, Edison R.; Pires, Carmen S. S.; Fontes, Eliana M. G.

Fuente: https://archive.org/







Documentos relacionados