Vol 41: The effect of various assisted hatching techniques on the mouse early embryo development.Reportar como inadecuado



 Vol 41: The effect of various assisted hatching techniques on the mouse early embryo development.


Vol 41: The effect of various assisted hatching techniques on the mouse early embryo development. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Descargar gratis o leer online en formato PDF el libro: Vol 41: The effect of various assisted hatching techniques on the mouse early embryo development.
This article is from Clinical and Experimental Reproductive Medicine, volume 41.AbstractObjective: In search of an ideal method of assisted hatching AH, we compared the effects of conventional micropipette-AH and laser-AH on the blastocyst formation rate BFR and blastocyst cell numbers. Methods: Four- to five-week-old ICR female mice were paired with male mice after superovulation using Pregnant mares serum gonadotropin PMSG and hCG. The two-cell embryos were flushed from the oviducts of female mice. The retrieved two-cell embryos underwent one of five AH procedures: single mechanical assisted hatching sMAH; cross mechanical assisted hatching cMAH; single laser assisted hatching sLAH; quarter laser assisted hatching qLAH; and quarter laser zona thinning assisted hatching qLZT-AH. After 72 hours incubation, double immunofluorescence staining was performed. Results: Following a 72 hours incubation, a higher hatching BFR was observed in the control, sMAH, cMAH, and sLAH groups, compared to those in the qLAH and qLZT-AH groups p



Autor: Park, Sung Baek; Kim, Hye Jin; Choi, Young Bae; Ahn, Kwang Hwa; Lee, Kee Hwan; Yang, Jung Bo; Yu, Chang Seok; Seo, Byoung Boo

Fuente: https://archive.org/







Documentos relacionados