Vol 4: Tear proteomic analysis of Sjgren syndrome patients with dry eye syndrome by two-dimensional-nano-liquid chromatography coupled with tandem mass spectrometry.Reportar como inadecuado



 Vol 4: Tear proteomic analysis of Sjgren syndrome patients with dry eye syndrome by two-dimensional-nano-liquid chromatography coupled with tandem mass spectrometry.


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This article is from Scientific Reports, volume 4.AbstractWe examined the tear film proteome of patients with Sjögrens syndrome SS and dry eye syndrome group A, patients with dry eye symptoms group B and normal volunteers group C. Tear samples were pooled from 8 subjects from each group and were subjected to two-dimensional-nano-liquid chromatography coupled with tandem mass spectrometry 2D-nano-LC-MS-MS. The tear breakup time for group A was significantly reduced compared with group B and C P 0.001. Group A Schirmer I test, 2.13 ± 2.38 mm-5 min had markedly lower tear volume than group B 5.94 ± 4.75 mm-5 min and C 14.44 ± 6.57 mm-5 min P 0.001. Group A had significantly higher normalized tear protein content 1.8291 ± 0.2241 μg-mm than group B 1.0839 ± 0.1120 μg-mm P = 0.001 and C 0.2028 ± 0.0177 μg-mm P = 0.001. The 2D-nano-LC-MS-MS analysis identified a total of 435 proteins, including 182 54.8%, 247 74.4% and 278 83.7% in group A, B, and C, respectively, with 56 16.7% proteins including defensin α1, clusterin and lactotransferrin unique to group A. In conclusion, dry eye syndrome in SS patients is associated with an altered proteomic profile with dysregulated expression of proteins involved in a variety of important cellular process including inflammation, immunity, and oxidative stress.



Autor: Li, Bing; Sheng, Minjie; Li, Jianhua; Yan, Guoquan; Lin, Anjuan; Li, Min; Wang, Weifang; Chen, Yihui

Fuente: https://archive.org/



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