Vol 42: Scp160p is required for translational efficiency of codon-optimized mRNAs in yeast.Reportar como inadecuado



 Vol 42: Scp160p is required for translational efficiency of codon-optimized mRNAs in yeast.


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This article is from Nucleic Acids Research, volume 42.AbstractThe budding yeast multi-K homology domain RNA-binding protein Scp160p binds to 1000 messenger RNAs mRNAs and polyribosomes, and its mammalian homolog vigilin binds transfer RNAs tRNAs and translation elongation factor EF1alpha. Despite its implication in translation, studies on Scp160ps molecular function are lacking to date. We applied translational profiling approaches and demonstrate that the association of a specific subset of mRNAs with ribosomes or heavy polysomes depends on Scp160p. Interaction of Scp160p with these mRNAs requires the conserved K homology domains 13 and 14. Transfer RNA pairing index analysis of Scp160p target mRNAs indicates a high degree of consecutive use of iso-decoding codons. As shown for one target mRNA encoding the glycoprotein Pry3p, Scp160p depletion results in translational downregulation but increased association with polysomes, suggesting that it is required for efficient translation elongation. Depletion of Scp160p also decreased the relative abundance of ribosome-associated tRNAs whose codons show low potential for autocorrelation on mRNAs. Conversely, tRNAs with highly autocorrelated codons in mRNAs are less impaired. Our data indicate that Scp160p might increase the efficiency of tRNA recharge, or prevent diffusion of discharged tRNAs, both of which were also proposed to be the likely basis for the translational fitness effect of tRNA pairing.



Autor: Hirschmann, Wolf D.; Westendorf, Heidrun; Mayer, Andreas; Cannarozzi, Gina; Cramer, Patrick; Jansen, Ralf-Peter

Fuente: https://archive.org/







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