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 Vol 27: Repurposing CRISPR-Cas9 for in situ functional assays.


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This article is from Genes & Development, volume 27.AbstractMalina et al. readapted the CRISPR-Cas9 genome-editing system for targeted gene disruption positive selection assays. They generated -all-in-one- lentiviral and retroviral delivery vectors heterologously expressing both a codon-optimized Cas9 and its synthetic guide RNA sgRNA and linked Cas9 expression to GFP fluorescence for in vivo applications. These results establish Cas9 genome editing as a powerful and practical approach for positive in situ genetic screens.



Autor: Malina, Abba; Mills, John R.; Cencic, Regina; Yan, Yifei; Fraser, James; Schippers, Laura M.; Paquet, Marilene; Dostie, Josee; Pelletier, Jerry

Fuente: https://archive.org/







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