Vol 9: Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization in the Half-Smooth Tongue Sole Cynoglossus semilaevis at Different Developmental Stages, in Various Tissue Types and on Exposure to Chemicals.Reportar como inadecuado



 Vol 9: Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization in the Half-Smooth Tongue Sole Cynoglossus semilaevis at Different Developmental Stages, in Various Tissue Types and on Exposure to Chemicals.


Vol 9: Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization in the Half-Smooth Tongue Sole Cynoglossus semilaevis at Different Developmental Stages, in Various Tissue Types and on Exposure to Chemicals. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

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This article is from PLoS ONE, volume 9.AbstractQuantitative real time RT-PCR has been described as the most sensitive method for the detection of low abundance mRNA. To date, no reference genes have been screened in the half-smooth tongue sole Cynoglossus semilaevis. The aim of this study was to select the most stable genes for quantitative real-time RT-PCR. Eight housekeeping genes 18S, TUBA, B2M, ACTB, EF1A, GAPDH, RPL17 and UBCE were tested at different developmental stages, in different tissues, and following exposure to the drug SB-431542. Using geNorm, BestKeeper and NormFinder software, GAPDH-B2M, GAPDH-18S and UBCE-GAPDH were identified as the most suitable genes from samples taken of different developmental stages while 18S-RPL17 were consistently ranked as the best reference genes for different tissue types. Furthermore, TUBA-B2M, TUBA-UBCE and B2M-TUBA were found to be the most suitable genes in samples treated with the drug, SB-431542 by geNorm, BestKeeper and NormFinder respectively. Across both different developmental stages and tissue types, the combination of 18S and GAPDH was the most stable reference gene analyzed by Ref-Finder. To test and verify the screened reference genes, the expression profiles of LEFTY-normalized to the combination of GAPDH-18S and ACTB were presented. These results will be useful for future gene-expression studies in the half-smooth tongue sole.



Autor: Liu, Conghui; Xin, Nian; Zhai, Yi; Jiang, Liming; Zhai, Jieming; Zhang, Quanqi; Qi, Jie

Fuente: https://archive.org/







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