Vol 9: Redistribution of Ionotropic Glutamate Receptors Detected by Laser Microdissection of the Rat Dentate Gyrus 48 h following LTP Induction In Vivo.Reportar como inadecuado



 Vol 9: Redistribution of Ionotropic Glutamate Receptors Detected by Laser Microdissection of the Rat Dentate Gyrus 48 h following LTP Induction In Vivo.


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This article is from PLoS ONE, volume 9.AbstractThe persistence and input specificity of long-term potentiation LTP make it attractive as a mechanism of information storage. In its initial phase, both in vivo and in vitro studies have shown that LTP is associated with increased membrane localization of AMPA receptor subunits, but the molecular basis of LTP maintenance over the long-term is still unclear. We have previously shown that expression of AMPA and NMDA receptor subunits is elevated in whole homogenates prepared from dentate gyrus 48 h after LTP induction in vivo. In the present study, we utilized laser microdissection LMD techniques to determine whether AMPA and NMDA receptor upregulation occurs specifically in the stimulated regions of the dentate gyrus dendritic arbor. Receptor proteins GluN1, GluA1 and GluA2, as well as postsynaptic density protein of 95 kDa and tubulin were detected by Western blot analysis in microdissected samples. Gradients of expression were observed for GluN1 and GluA2, decreasing from the inner to the outer zones of the molecular layer, and were independent of LTP. When induced at medial perforant path synapses, LTP was associated with an apparent specific redistribution of GluA1 and GluN1 to the middle molecular layer that contains these synapses. These data indicate that glutamate receptor proteins are delivered specifically to dendritic regions possessing LTP-expressing synapses, and that these changes are preserved for at least 48 h.



Autor: Kennard, Jeremy T. T.; Guevremont, Diane; Mason-Parker, Sara E.; Abraham, Wickliffe C.; Williams, Joanna M.

Fuente: https://archive.org/







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