Vol 9: RNA Sequence Reveals Mouse Retinal Transcriptome Changes Early after Axonal Injury.Reportar como inadecuado



 Vol 9: RNA Sequence Reveals Mouse Retinal Transcriptome Changes Early after Axonal Injury.


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This article is from PLoS ONE, volume 9.AbstractGlaucoma is an ocular disease characterized by progressive retinal ganglion cell RGC death caused by axonal injury. However, the underlying mechanisms involved in RGC death remain unclear. In this study, we investigated changes in the transcriptome profile following axonal injury in mice C57BL-6 with RNA sequencing RNA-seq technology. The experiment group underwent an optic nerve crush ONC procedure to induce axonal injury in the right eye, and the control group underwent a sham procedure. Two days later, we extracted the retinas and performed RNA-seq and a pathway analysis. We identified 177 differentially expressed genes with RNA-seq, notably the endoplasmic reticulum ER stress-related genes Atf3, Atf4, Atf5, Chac1, Chop, Egr1 and Trb3, which were significantly upregulated. The pathway analysis revealed that ATF4 was the most significant upstream regulator. The antioxidative response-related genes Hmox1 and Srxn1, as well as the immune response-related genes C1qa, C1qb and C1qc, were also significantly upregulated. To our knowledge, this is the first reported RNA-seq investigation of the retinal transcriptome and molecular pathways in the early stages after axonal injury. Our results indicated that ER stress plays a key role under these conditions. Furthermore, the antioxidative defense and immune responses occurred concurrently in the early stages after axonal injury. We believe that our study will lead to a better understanding of and insight into the molecular mechanisms underlying RGC death after axonal injury.



Autor: Yasuda, Masayuki; Tanaka, Yuji; Ryu, Morin; Tsuda, Satoru; Nakazawa, Toru

Fuente: https://archive.org/







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