Natural co-infection of solanum tuberosum crops by the potato yellow vein virus and potyvirus in colombia Reportar como inadecuado




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The Potato yellow vein virus PYVV, a Crinivirus with an RNA tripartite genome, is the causal agent of the potato yellow vein disease, reported in Colombian since 1950, with yield reductions of up to 50%. Co-infection of two or more viruses is common in nature and can be associated with differences in virus accumulation and symptom expression. No evidence of mixed infection between PYVV and other viruses has been reported. In this study, eight plants showing yellowing PYVV symptoms: four Solanum tuberosum Group Phureja P and four Group Andigena A, were collected in Cundinamarca, Colombia to detect mixed infection in the isolates using next generation sequencing NGS. The Potato virus Y PVY complete genome similar to N strain and the Potato virus V PVV partial genomes were detected using NGS and re-confirmed by RT-PCR. Preliminary field screening in a large sample showed that PYVV and PVY co-infect potato plants with a prevalence of 21% within the P group and 23% within the A group. This is the first report of co-infection of PYVV and potyvirus in Colombia and with the use of NGS. Considering that potyviruses enhance symptom severity and-or yield reductions in mixed infections, our results may be relevant for disease diagnosis, breeding programs and tuber certification.

Tipo de documento: Artículo - Article

Información adicional: © Facultad de Agronomía, Universidad Nacional de Colombia Se autoriza la reproducción y citación del material que aparece en la revista, siempre y cuando se indique de manera explícita: nombre de la revista, nombre del autores, año, volumen, número y páginas del artículo fuente. Las ideas y afirmaciones consignadas por los autores están bajo su responsabilidad y no interpretan necesariamente las opiniones y políticas de la Universidad Nacional de Colombia. La mención de productos o firmas comerciales en la revista no implica una recomendación o apoyo por parte de la Universidad ni de la Facultad; el uso de tales productos debe ceñirse a las recomendaciones de las etiquetas.

Palabras clave: Viral diseases, Potato virus V, prevalence, NGS, potato, root vegetables., Plant virology, potatoes





Fuente: http://www.bdigital.unal.edu.co


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crop protection Natural co-infection of Solanum tuberosum crops by the Potato yellow vein virus and potyvirus in Colombia Co-infección natural de Potato yellow vein virus y potivirus en cultivos de Solanum tuberosum en Colombia Angela Villamil-Garzón1, Wilmer J.
Cuellar2, and Mónica Guzmán-Barney1 ABSTRACT RESUMEN The Potato yellow vein virus (PYVV), a Crinivirus with an RNA tripartite genome, is the causal agent of the potato yellow vein disease, reported in Colombian since 1950, with yield reductions of up to 50%.
Co-infection of two or more viruses is common in nature and can be associated with differences in virus accumulation and symptom expression.
No evidence of mixed infection between PYVV and other viruses has been reported.
In this study, eight plants showing yellowing PYVV symptoms: four Solanum tuberosum Group Phureja (P) and four Group Andigena (A), were collected in Cundinamarca, Colombia to detect mixed infection in the isolates using next generation sequencing (NGS).
The Potato virus Y (PVY) complete genome (similar to N strain) and the Potato virus V (PVV) partial genomes were detected using NGS and re-confirmed by RT-PCR.
Preliminary field screening in a large sample showed that PYVV and PVY co-infect potato plants with a prevalence of 21% within the P group and 23% within the A group.
This is the first report of co-infection of PYVV and potyvirus in Colombia and with the use of NGS.
Considering that potyviruses enhance symptom severity and-or yield reductions in mixed infections, our results may be relevant for disease diagnosis, breeding programs and tuber certification. El Potato yellow vein virus (PYVV) es un Crinivirus de genoma RNA tripartita, agente causal de la enfermedad del amarillamiento de las nervaduras de la papa reportada desde 1950 en Colombia con reducción de producción hasta 50%. La coinfección entre virus en un mismo hospedero es común en la naturaleza y se asocia con cambios en acumulación viral y en la expresión ...






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