Vol 9: PolyC-Binding Protein 1 Interacts with 5-Untranslated Region of Enterovirus 71 RNA in Membrane-Associated Complex to Facilitate Viral Replication.Reportar como inadecuado



 Vol 9: PolyC-Binding Protein 1 Interacts with 5-Untranslated Region of Enterovirus 71 RNA in Membrane-Associated Complex to Facilitate Viral Replication.


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This article is from PLoS ONE, volume 9.AbstractEnterovirus 71 EV71 is one causative agent of hand, foot, and mouth disease HFMD, which may lead to severe neurological disorders and mortality in children. EV71 genome is a positive single-stranded RNA containing a single open reading frame ORF flanked by 5′-untranslated region 5′UTR and 3′UTR. The 5′UTR is fundamentally important for virus replication by interacting with cellular proteins. Here, we revealed that polyC-binding protein 1 PCBP1 specifically binds to the 5′UTR of EV71. Detailed studies indicated that the RNA-binding K-homologous 1 KH1 domain of PCBP1 is responsible for its binding to the stem-loop I and IV of EV71 5′UTR. Interestingly, we revealed that PCBP1 is distributed in the nucleus and cytoplasm of uninfected cells, but mainly localized in the cytoplasm of EV71-infected cells due to interaction and co-localization with the viral RNA. Furthermore, sub-cellular distribution analysis showed that PCBP1 is located in ER-derived membrane, in where virus replication occurred in the cytoplasm of EV71-infected cells, suggesting PCBP1 is recruited in a membrane-associated replication complex. In addition, we found that the binding of PCBP1 to 5′UTR resulted in enhancing EV71 viral protein expression and virus production so as to facilitate viral replication. Thus, we revealed a novel mechanism in which PCBP1 as a positive regulator involved in regulation of EV71 replication in the host specialized membrane-associated replication complex, which provides an insight into cellular factors involved in EV71 replication.



Autor: Luo, Zhen; Dong, Xingchen; Li, Youxing; Zhang, Qi; Kim, Cholho; Song, Yu; Kang, Lei; Liu, Yingle; Wu, Kailang; Wu, Jianguo

Fuente: https://archive.org/







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