Vol 15: Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes.Report as inadecuate



 Vol 15: Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes.


Vol 15: Purification and Characterization of Aporphine Alkaloids from Leaves of Nelumbo nucifera Gaertn and Their Effects on Glucose Consumption in 3T3-L1 Adipocytes. - Download this document for free, or read online. Document in PDF available to download.

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This article is from International Journal of Molecular Sciences, volume 15.AbstractAporphine alkaloids from the leaves of Nelumbo nucifera Gaertn are substances of great interest because of their important pharmacological activities, particularly anti-diabetic, anti-obesity, anti-hyperlipidemic, anti-oxidant, and anti-HIV’s activities. In order to produce large amounts of pure alkaloid for research purposes, a novel method using high-speed counter-current chromatography HSCCC was developed. Without any initial cleanup steps, four main aporphine alkaloids, including 2-hydroxy-1-methoxyaporphine, pronuciferine, nuciferine and roemerine were successfully purified from the crude extract by HSCCC in one step. The separation was performed with a simple two-phase solvent system composed of n-hexane-ethyl acetate-methanol-acetonitrile-water 5:3:3:2.5:5, v-v-v-v-v. In each operation, 100 mg crude extracts was separated and yielded 6.3 mg of 2-hydroxy-1-methoxyaporphine 95.1% purity, 1.1 mg of pronuciferine 96.8% purity, 8.5 mg of nuciferine 98.9% purity, and 2.7 mg of roemerine 97.4% respectively. The chemical structure of four aporphine alkaloids are identified by means of electrospray ionization MS ESI-MS and nuclear magnetic resonance NMR analysis. Moreover, the effects of four separated aporphine alkaloids on insulin-stimulated glucose consumption were examined in 3T3-L1 adipocytes. The results showed that 2-hydroxy-1-methoxyaporphine and pronuciferine increased the glucose consumption significantly as rosiglitazone did.



Author: Ma, Chengjun; Wang, Jinjun; Chu, Hongmei; Zhang, Xiaoxiao; Wang, Zhenhua; Wang, Honglun; Li, Gang

Source: https://archive.org/







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