Vol 9: PolyTrimethylene Carbonate-co-Caprolactone Promotes Axonal Growth.Report as inadecuate



 Vol 9: PolyTrimethylene Carbonate-co-Caprolactone Promotes Axonal Growth.


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This article is from PLoS ONE, volume 9.AbstractMammalian central nervous system CNS neurons do not regenerate after injury due to the inhibitory environment formed by the glial scar, largely constituted by myelin debris. The use of biomaterials to bridge the lesion area and the creation of an environment favoring axonal regeneration is an appealing approach, currently under investigation. This work aimed at assessing the suitability of three candidate polymers – polyε-caprolactone, polytrimethylene carbonate-co-ε-caprolactone PTMC-CL 11∶89 mol% and polytrimethylene carbonate - with the final goal of using these materials in the development of conduits to promote spinal cord regeneration. PolyL-lysine PLL coated polymeric films were tested for neuronal cell adhesion and neurite outgrowth. At similar PLL film area coverage conditions, neuronal polarization and axonal elongation was significantly higher on PTMC-CL films. Furthermore, cortical neurons cultured on PTMC-CL were able to extend neurites even when seeded onto myelin. This effect was found to be mediated by the glycogen synthase kinase 3β GSK3β signaling pathway with impact on the collapsin response mediator protein 4 CRMP4, suggesting that besides surface topography, nanomechanical properties were implicated in this process. The obtained results indicate PTMC-CL as a promising material for CNS regenerative applications as it promotes axonal growth, overcoming myelin inhibition.



Author: Rocha, Daniela Nogueira; Brites, Pedro; Fonseca, Carlos; Pego, Ana Paula

Source: https://archive.org/







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