Vol 14: Par6 is an essential mediator of apoptotic response to transforming growth factor beta in NMuMG immortalized mammary cells.Report as inadecuate



 Vol 14: Par6 is an essential mediator of apoptotic response to transforming growth factor beta in NMuMG immortalized mammary cells.


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This article is from Cancer Cell International, volume 14.AbstractBackground: We previously observed that the TGFbeta-Par6 pathway mediates loss of polarity and apoptosis in NMuMG cells. Here we investigate the contribution of Par6 versus TGFbeta receptor I activation to TGFbeta-induced apoptosis in association with changes in apico-basal polarity. We focus on the effect of Par6 activation on alpha6beta4 integrin expression and localization, and Nuclear Factor-kappaB p65-RelA activation, previously shown to mediate polarity-dependent cell survival. Methods: Using immunoblotting and-or immunofluorescence we investigated the effect of TGFbeta1 on apoptosis, alpha6, beta4 and beta1 integrin expression-localization, and p65-RelA phosphorylation-localization in monolayer and three-dimensional 3D cultures of NMuMG cells with an overactive or inactive Par6 pathway. Results were quantified by band densitometry or as percent of 3D structures displaying a phenotype. Differences among means were compared by two-way ANOVA. Results: Blocking Par6 activation inhibits TGFbeta-induced apoptosis. Par6 overactivation enhances TGFbeta-induced apoptosis, notably after 6-day exposure to TGFbeta p 



Author: Avery-Cooper, Geordon; Doerr, Meghan; Gilbert, Richard WD; Youssef, Mahmoud; Richard, Amy; Huether, Patricia; Viloria-Petit, Alicia M

Source: https://archive.org/







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