Segregation analysis of molecular markers in a population derived from coffea liberica hiern x c. eugenioides l Report as inadecuate




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Coffee is a very important crop for the world economy. The commercial coffee production is based on two species, Coffea arabica L. 70% and C. canephora Pierre 30%. C. arabica is the only allotetraploid 2n=4x=44 species, which has its primary center of diversity in the southwestern Ethiopia highlands. C. arabica is the only species cultivated in Colombia and represents a very important economic and social value. However, C. arabica has a very narrow genetic diversity and therefore, the use of diploid species on coffee breeding is very important because they allow to broad its diversity. In this work, the genetic segregation patterns were evaluated on a population of 101 F1 hybrid plants from a cross between the diploid species C. liberica and C. eugenioides using 618 molecular markers, of which 168 SSRs and 2 ESTs exhibited polymorphic patterns that allowed segregation analysis. Approximately 24% of the loci were null alleles, and the segregation distortion reached 23.5% at 0.01. A total of four segregant types were observed, from up to seven possibilities. The origins of null alleles, possible causes of segregation distortion, different segregation patterns obtained and recombination frequencies are discussed. The knowledge derived from this study allowed to better understand the genetic segregation behavior of these markers with the aim of developing genetic maps that have important applications for coffee breeding programs as well as other commercial crops., El café es un cultivo importante para la economía mundial. La producción comercial de café se basa en dos especies, Coffea arabica L. 70% y C. canephora Pierre 30%. La primera es la única alotetraploide 2n = 4x = 44 que tiene su centro primario de diversidad en las tierras altas del suroeste de Etiola segunda es la única cultivada en Colombia y representa un importante valor económico y social. Sin embargo, C. arabica tiene una base genética muy estrecha y por ello es importante el uso de especies diploides en el mejora-miento del café para ampliar su diversidad. En este trabajo fueron evaluados los patrones de segregación genética en una población consistente en 101 plantas híbridas F1 de un cruce entre la especie diploide C. liberica y C. eugenioides utilizando 618 marcadores moleculares, de las cuales 168 SSR y 2 EST exhibieron patrones polimórficos que permitieron el análisis de segregación. Aproximadamente 24% de los loci fueron alelos nulos y la distorsión de la segregación alcanzó 23.5% a

Tipo de documento: Artículo - Article

Palabras clave: coffee, microsatellites, segregation distortion, Plant Breeding, Biotechnology, Café, microsatélites, distorsión de segregación.





Source: http://www.bdigital.unal.edu.co


Teaser



Segregation analysis of molecular markers in a population derived from Coffea liberica Hiern x C.
eugenioides L. Análisis de segregación de marcadores moleculares en una población derivada de Coffea liberica Hiern x C.
eugenioides L. 1Germán Ariel López Gartner*, 2Susan R.
McCouch and 3 and María Del Pilar Moncada Botero Universidad de Caldas, Calle 65 Nº 26-10, Manizales, Caldas, Colombia; 2Susan R.
McCouch, Department of Plant Breeding, Emerson Hall, Cornell University, Ithaca, Nueva York 14853, USA; 3Centro Nacional de Investigaciones de Café, Mejoramiento Genético, Cenicafé, Chinchiná, Caldas, Colombia.
*Corresponding author: german.lopez@ucaldas.edu.co, Telephone: (57) 68781500 ext.
12189, Fax (57) 68781500 ext.
12194 1 Rec.: 09.13.2013 Acep.: 01.27.2014 Abstract Coffee is a very important crop for the world economy.
The commercial coffee production is based on two species, Coffea arabica L.
(70%) and C.
canephora Pierre (30%).
C.
arabica is the only allotetraploid (2n=4x=44) species, which has its primary center of diversity in the southwestern Ethiopia highlands.
C. arabica is the only species cultivated in Colombia and represents a very important economic and social value.
However, C.
arabica has a very narrow genetic diversity and therefore, the use of diploid species on coffee breeding is very important because they allow to broad its diversity.
In this work, the genetic segregation patterns were evaluated on a population of 101 F1 hybrid plants from a cross between the diploid species C.
liberica and C.
eugenioides using 618 molecular markers, of which 168 SSRs and 2 ESTs exhibited polymorphic patterns that allowed segregation analysis.
Approximately 24% of the loci were null alleles, and the segregation distortion reached 23.5% at   0.01.
A total of four segregant types were observed, from up to seven possibilities.
The origins of null alleles, possible causes of segregation distortion, different segregation patterns obtained and rec...






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