Identification of plant parasitic nematodes in the germplasm bank of yellow passion fruit (passiflora edulis f. flavicarpa degener) in colombia Reportar como inadecuado




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Plant parasitic nematodes are one of the major constraints in tropical fruit causing great economic losses by reducing yields. In Colombia there is not information dealing with the production systems about these parasites affecting passion fruit cultivation so, it is necessary to generate information for management strategies. The aim of this study was to identify and quantify plant parasitic nematodes present in the National yellow passion fruit Collection consisting of 28 accessions from different origin and established in the farm Luker in Palestina Caldas, Colombia. Sample collection was carried out in the root area of each plant at a depth of 60 cm in each accession. Three samples of 100 g of functional root system and 100 g of soil were taken. The extraction of root and soil nematodes was based on the flotation principle of nematodes in sugar. For data an univariate analysis with the estimated average, the coefficient of variation, analysis of variance and Tukey test at 5%, to establish the differences among accessions was performed. Results showed seven kinds of soil and root phytonematodes: Helicotylenchus, Rotylenchulus, Radopholus, Meloidogyne, Tylenchus, Aphelenchoides and Trichodorus. The species most frequently found in root and soil of accessions were Helicotylenchus dihystera, Rotylenchulus reniformis and Radopholus similis with 100, 75 and 61%, respectively. Results showed 11, 8 y 16 accessions without parasitism of the genera Radopholus, Rotylenchulus and Meloidogyne, respectively. Among these, accession AntFla03 and CauFla01 were outstanding due to their agronomic characteristics which constitute a gene pool for breeding programs in search for phytoparasitic nematode resistance or tolerance that involve hybridization or grafting., Los nematodos fitoparásitos son una de las mayores limitantes en la fruticultura tropical causando grandes pérdidas económicas por la reducción de los rendimientos. En Colombia no existe información respecto a estos parásitos que afectan el cultivo del maracuyá y es necesario generarla para crear estrategias de manejo. El objetivo del estudio fue identificar y cuantificar los nematodos fitoparásitos presentes en plantas de maracuyá de la Colección Nacional, conformada por 28 accesiones de diferentes procedencias establecida en la Granja Luker en Palestina, Caldas Colombia, a 1058 m.s.n.m., 5° 4 13.2- N y -75° 41 7.7- O, 23 °C, 2250 mm-ao y 75% de HR. La recolección de las muestras se realizó en la zona de plateo de cada planta hasta 60 cm de profundidad. En cada accesión se tomaron tres muestras de 100 g de raíces funcionales y 100 g de suelo. La extracción de nematodos de raíces y suelo se realizó con base en el principio de flotación en azúcar. Para los datos se desarrolló un análisis univariado con la estimación del promedio, el coeficiente de variación, el análisis de varianza y prueba de Tukey al 5%, para establecer las diferencias entre accesiones. Se recuperaron nematodos de siete géneros, Helicotylenchus, Rotylenchulus, Radopholus, Meloidogyne, Tylenchus, Aphelenchoides y Trichodorus. Las especies con mayor frecuencia en las accesiones, tanto en raíces como en suelo, fueron Helicotylenchus dihystera, Rotylenchulus reniformis y Radopholus similis con 100, 75 y 61%, respectivamente. Se encontraron 11, 8 y 16 accesiones de maracuyá sin parasitismo de los géneros Radopholus, Rotylenchulus y Meloidogyne, respectivamente. Dentro de éstas, sobresalen las accesiones AntFla03 y CauFla01 por sus características agronómicas, las cuales constituyen un reservorio genético para programas de fitomejoramiento en la búsqueda de resistencia o tolerancia a nematodos fitoparásitos que involucren hibridación o injertación.

Tipo de documento: Artículo - Article

Palabras clave: Colombia, fitonematodos, Helicotylenchus, maracuyá, nematodos de las plantas, Passiflora edulis, Radopholus similis, otylenchulus., Colombia, Helicotylenchus, maracuyá, Passiflora edulis, phytonematodes, plant nematodes, Radopholus similis, Rotylenchulus.





Fuente: http://www.bdigital.unal.edu.co


Introducción



Identification of plant parasitic nematodes in the Germplasm Bank of yellow passion fruit (Passiflora edulis f.
flavicarpa Degener) in Colombia Identificación de nematodos fitoparásitos en el Banco de Germoplasma de maracuyá (Passiflora edulis f.
flavicarpa Degener) en Colombia Rocío Alexandra Ortiz-Paz1†, Óscar Adrián Guzmán-Piedrahita2*, y John A.
Ocampo3‡ 1 Agroforestal Engineer, Master student in Phytopathology, Universidad de Caldas, Manizales, Caldas, Colombia.
2Agronomist Engineer, Ms.
in Phytopathology.
Auxiliar Professor, Departament of Agricultural Production, Faculty of Agricultural Sciences, Universidad de Caldas, Manizales, Caldas, Colombia.
3Agronomist Engineer., Ph.D.
Associated Professor, Universidad Nacional de Colombia - Palmira-International Center for Tropical Agriculture – CIAT, Colombia.
*Corresponding author: oscar.guzman@ucaldas.edu.co; †roalorpaz@hotmail.com; ‡jaocampop@unal.edu.co Rec.: 30.07.11 Acept.: 30.11.12 Abstract Plant parasitic nematodes are one of the major constraints in tropical fruit causing great economic losses by reducing yields.
In Colombia there is not information dealing with the production systems about these parasites affecting passion fruit cultivation so, it is necessary to generate information for management strategies.
The aim of this study was to identify and quantify plant parasitic nematodes present in the National yellow passion fruit Collection consisting of 28 accessions from different origin and established in the farm Luker in Palestina (Caldas), Colombia.
Sample collection was carried out in the root area of each plant at a depth of 60 cm in each accession.
Three samples of 100 g of functional root system and 100 g of soil were taken.
The extraction of root and soil nematodes was based on the flotation principle of nematodes in sugar.
For data an univariate analysis with the estimated average, the coefficient of variation, analysis of variance and Tukey test at 5%, to establish the diffe...






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