Vol 55: Perinatal hypoxia: different effects of the inhibitors of GABA transporters GAT1 and GAT3 on the initial velocity of 3HGABA uptake by cortical, hippocampal, and thalamic nerve terminals.Reportar como inadecuado



 Vol 55: Perinatal hypoxia: different effects of the inhibitors of GABA transporters GAT1 and GAT3 on the initial velocity of 3HGABA uptake by cortical, hippocampal, and thalamic nerve terminals.


Vol 55: Perinatal hypoxia: different effects of the inhibitors of GABA transporters GAT1 and GAT3 on the initial velocity of 3HGABA uptake by cortical, hippocampal, and thalamic nerve terminals. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

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This article is from Croatian Medical Journal, volume 55.AbstractAim: To analyze the effects of highly selective blocker GAT1, NO-711, and substrate inhibitor GAT3, β-alanine, on the initial velocity of 3HGABA uptake by cortical, hippocampal, and thalamic nerve terminals synaptosomes after perinatal hypoxia. Methods: Animals were divided into two groups: control n = 17 and hypoxia n = 12. Rats in the hypoxia group underwent hypoxia and seizures airtight chamber, 4% O2 and 96% N2 at the age of 10-12 postnatal days and were used in the experiments 8-9 weeks after hypoxia. Results: In cortical synaptosomes, the effects of NO-711 30 μΜ and β-alanine 100 μΜ on 3HGABA uptake were similar in control and hypoxia groups. In hippocampal synaptosomes, NO-711 inhibited 84.3% of the initial velocity of 3HGABA uptake in normal conditions and 80.1% after hypoxia, whereas the effect of β-alanine was increased after hypoxia from 14.4% to 22.1%. In thalamic synaptosomes, the effect of NO-711 was decreased by 79.6% in controls and by 70.9% in hypoxia group, whereas the effect of β-alanine was increased after hypoxia from 20.2% to 30.2%. Conclusions: The effectiveness of β-alanine to influence GABA uptake was increased in hippocampal and thalamic nerve terminals as a result of perinatal hypoxia and the effectiveness of NO-711 in thalamic nerve terminals was decreased. These results may indicate changes in the ratio of active GAT1-GAT3 expressed in the plasma membrane of nerve terminals after perinatal hypoxia. We showed a possibility to modulate non-GAT1 GABA transporter activity in different brain regions by exogenous and endogenous β-alanine.



Autor: Pozdnyakova, Natalia; Dudarenko, Marina; Yatsenko, Ludmila; Himmelreich, Nina; Krupko, Olga; Borisova, Tatiana

Fuente: https://archive.org/



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