Vol 9: Patterns of Gene Expression Associated with Pten Deficiency in the Developing Inner Ear.Reportar como inadecuado



 Vol 9: Patterns of Gene Expression Associated with Pten Deficiency in the Developing Inner Ear.


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This article is from PLoS ONE, volume 9.AbstractIn inner ear development, phosphatase and tensin homolog PTEN is necessary for neuronal maintenance, such as neuronal survival and accurate nerve innervations of hair cells. We previously reported that Pten conditional knockout cKO mice exhibited disorganized fasciculus with neuronal apoptosis in spiral ganglion neurons SGNs. To better understand the genes and signaling networks related to auditory neuron maintenance, we compared the profiles of differentially expressed genes DEGs using microarray analysis of the inner ear in E14.5 Pten cKO and wild-type mice. We identified 46 statistically significant transcripts using significance analysis of microarrays, with the false-discovery rate set at 0%. Among the DEGs, expression levels of candidate genes and expression domains were validated by quantitative real-time RT-PCR and in situ hybridization, respectively. Ingenuity pathway analysis using DEGs identified significant signaling networks associated with apoptosis, cellular movement, and axon guidance i.e., secreted phosphoprotein 1 Spp1-mediated cellular movement and regulator of G-protein signaling 4 Rgs4-mediated axon guidance. This result was consistent with the phenotypic defects of SGNs in Pten cKO mice e.g., neuronal apoptosis, abnormal migration, and irregular nerve fiber patterns of SGNs. From this study, we suggest two key regulatory signaling networks mediated by Spp1 and Rgs4, which may play potential roles in neuronal differentiation of developing auditory neurons.



Autor: Kim, Hyung Jin; Ryu, Jihee; Woo, Hae-Mi; Cho, Samuel Sunghwan; Sung, Min Kyung; Kim, Sang Cheol; Park, Mi-Hyun; Park, Taesung; Koo, Soo Kyung

Fuente: https://archive.org/







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