Vol 34: Proliferation and odontogenic differentiation of BMP2 gene-transfected stem cells from human tooth apical papilla: An in vitro study.Reportar como inadecuado



 Vol 34: Proliferation and odontogenic differentiation of BMP2 gene-transfected stem cells from human tooth apical papilla: An in vitro study.


Vol 34: Proliferation and odontogenic differentiation of BMP2 gene-transfected stem cells from human tooth apical papilla: An in vitro study. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

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This article is from International Journal of Molecular Medicine, volume 34.AbstractStem cells from the apical papilla SCAP have odontogenic potential, which plays a pivotal role in the root dentin development of permanent teeth. Human bone morphogenetic protein 2 BMP2 is a well-known gene that participates in regulating the odontogenic differentiation of dental tissue-derived stem cells. However, little is known regarding the effects of the BMP2 gene on the proliferation and odontogenic differentiation of SCAP. This study aimed to evaluate the odontogenic differentiation potential of lentiviral-mediated BMP2 gene-transfected human SCAP SCAP-BMP2 in vitro. SCAP were isolated by enzymatic dissociation of human teeth apical papillae. The multipotential of SCAP was verified by their osteogenic and adipogenic differentiation characteristics. The phenotype of SCAP was evaluated by flow cytometry FCM. The proliferation status of the blank vector-transfected SCAP SCAP-Vector and SCAP-BMP2 was analyzed by a cell counting kit-8 CCK-8. Odontogenic genes, including alkaline phosphatase ALP, osteocalcin OCN, dentin sialophosphoprotein DSPP and dentin matrix protein 1 DMP1 of the two groups of cells were evaluated by quantitative polymerase chain reaction qPCR. ALP staining and alizarin red AR staining of the cells was performed on the 16th day after transfection. In vitro results of CCK-8, qPCR, ALP and AR staining demonstrated that: i SCAP-BMP2 had a comparable proliferation rate to SCAP-Vector; ii SCAP-BMP2 presented significantly better potential to differentiate into odontoblasts compared to SCAP-Vector by upregulating ALP, OCN, DSPP and DMP1 genes; iii more ALP granules and mineralized deposits were formed by SCAP-BMP2 as compared to SCAP-Vector. The results suggested that lentiviral-mediated BMP2 gene transfection enhances the odontogenic differentiation capacity of human SCAP in vitro.



Autor: ZHANG, WEN; ZHANG, XIAOLEI; LING, JUNQI; LIU, WEI; ZHANG, XINCHUN; MA, JINGLEI; ZHENG, JIANMAO

Fuente: https://archive.org/







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