Vol 8: Ptaquiloside-induced cytotoxicity in Crandall feline kidney and HGC-27 cells.Reportar como inadecuado



 Vol 8: Ptaquiloside-induced cytotoxicity in Crandall feline kidney and HGC-27 cells.


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This article is from Oncology Letters, volume 8.AbstractPtaquiloside PTA is a potent genotoxic carcinogenic compound, which is found in bracken ferns and predominantly causes gastric tumors in humans, as well as bladder tumors and chronic enzootic hematuria in cattle. The underlying molecular mechanisms of PTA remain a topic for interdisciplinary investigation. The aim of the present study was to determine the possible cytotoxic effect of 24 h of PTA exposure in Crandall feline kidney CrFK and human gastric cells the HGC-27 cell line using the 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide MTT assay and lactose dehydrogenase LDH analysis. The cytotoxic effects of PTA 0.0005–500 μg-ml were found to increase in a dose-dependent manner, whereby the half maximal inhibitory concentration values were 11.17 and 11.86 μg-ml in the CrFK cells, and 2.03 and 2.56 μg-ml in the HGC-27 cells, by LDH and MTT assay, respectively. The results of the present study are consistent with those of previous studies associated with the cytotoxicity of PTA; however, cytotoxicity was identified to occur at significantly lower doses. This cytotoxic effect in vitro at particularly high doses may be linked to the initiation of carcinogenesis as a result of oxidative stress.



Autor: YURDAKOK, BEGUM; KISMALI, GORKEM; OZEN, DOGUKAN

Fuente: https://archive.org/







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