Vol 47: Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1 expression.Reportar como inadecuado



 Vol 47: Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1 expression.


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This article is from Brazilian Journal of Medical and Biological Research, volume 47.AbstractHypoxia-inducible factor-1α HIF-1α is one of the most potent angiogenic growth factors. It improves angiogenesis and tissue perfusion in ischemic skeletal muscle. In the present study, we tested the hypothesis that ischemic postconditioning is effective for salvaging ischemic skeletal muscle resulting from limb ischemia-reperfusion injury, and that the mechanism involves expression of HIF-1α. Wistar rats were randomly divided into three groups n=36 each: sham-operated group S, hindlimb ischemia-reperfusion group IR, and ischemic postconditioning group IPO. Each group was divided into subgroups n=6 according to reperfusion time: immediate 0 h, T0, 1 h T1, 3 h T3, 6 h T6, 12 h T12, and 24 h T24. In the IPO group, three cycles of 30-s reperfusion and 30-s femoral aortic reocclusion were carried out before reperfusion. At all reperfusion times T0-T24, serum creatine kinase CK and lactate dehydrogenase LDH activities, as well as interleukin IL-6, IL-10, and tumor necrosis factor-α TNF-α concentrations, were measured in rats after they were killed. Histological and immunohistochemical methods were used to assess the skeletal muscle damage and HIF-1α expression in skeletal muscle ischemia. In groups IR and IPO, serum LDH and CK activities and TNF-α, IL-6, and IL-10 concentrations were all significantly increased compared to group S, and HIF-1α expression was up-regulated P



Autor: Wang, T.; Zhou, Y.T.; Chen, X.N.; Zhu, A.X.

Fuente: https://archive.org/







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