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Recycled paper sludge is a promising raw material for ethanol production. In this study, we first evaluated the effects of ethanol concentration, solids load, and cellulose crystallinity on the enzymatic hydrolysis of cellulose to produce reducing sugars. We then evaluated the production of ethanol by either saccharification and simultaneous fermentation SSF or separated hydrolysis and fermentation SHF using the yeast Kluyveromyces marxianus ATCC 36907. We found that cellulose hydrolysis decreased as ethanol concentrations increased; at 40 g-L ethanol, the reducing sugar production was decreased by 79 %. Hydrolysis also decreased as solids load increased; at 9 % of solids, the cellulose conversion was 76 % of the stoichiometric production. The ethanol yield and cellulose conversion rate were higher with SSF as opposed to SHF processes at 72 h of treatment.

Tipo de documento: Artículo - Article

Información adicional: Derechos de autor reservados

Palabras clave: Paper sludge, ethanol, Kluyveromyces marxianus, fermentation, hydrolysis





Fuente: http://www.bdigital.unal.edu.co


Introducción



ethanol production from paper sludge using Kluyveromyces marxianus Producción de etanol de lodos papeleros USANDO Kluyveromyces marxianus LINA MARÍA MADRID Ingeniera Química.
Chemical Engineering Department, Universidad de Antioquia, lunanita@gmail.com JUAN CARLOS QUINTERO DÍAZ Doctor en ingeniería.
Química.
Chemical Engineering Department, Associate Professor, Universidad de Antioquia, jcquinte@udea.edu.co Received for review: September 9th, 2009; accepted: September 13rd, 2010; final version: October 14nd, 2010 ABSTRACT: Recycled paper sludge is a promising raw material for ethanol production.
In this study, we first evaluated the effects of ethanol concentration, solids load, and cellulose crystallinity on the enzymatic hydrolysis of cellulose to produce reducing sugars.
We then evaluated the production of ethanol by either saccharification and simultaneous fermentation (SSF) or separated hydrolysis and fermentation (SHF) using the yeast Kluyveromyces marxianus ATCC 36907.
We found that cellulose hydrolysis decreased as ethanol concentrations increased; at 40 g-L ethanol, the reducing sugar production was decreased by 79 %.
Hydrolysis also decreased as solids load increased; at 9 % of solids, the cellulose conversion was 76 % of the stoichiometric production.
The ethanol yield and cellulose conversion rate were higher with SSF as opposed to SHF processes at 72 h of treatment. KEYWORDS: Paper sludge, ethanol, Kluyveromyces marxianus, fermentation, hydrolysis RESUMEN: El lodo de papel reciclado es una materia prima promisoria para la producción de etanol.
En esta investigación, nosotros primero evaluamos el efecto de la concentración de etanol, la carga de sólidos y la cristalinidad de la celulosa sobre la hidrólisis enzimática de la celulosa.
También se evaluó la producción de etanol con lodo de papel mediante el proceso de sacarificación y fermentación simultanea (SSF) e hidrólisis y fermentación separada (SHF) empleando la levadura Kluyveromy...






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