Vol 13: Lactobacillus acidophilus CRL 1014 improved gut health in the SHIME® reactor.Reportar como inadecuado



 Vol 13: Lactobacillus acidophilus CRL 1014 improved gut health in the SHIME® reactor.


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This article is from BMC Gastroenterology, volume 13.AbstractBackground: How to maintain -gut health- is a goal for scientists throughout the world. Therefore, microbiota management models for testing probiotics, prebiotics, and synbiotics have been developed. Methods: The SHIME® model was used to study the effect of Lactobacillus acidophilus 1014 on the fermentation pattern of the colon microbiota. Initially, an inoculum prepared from human feces was introduced into the reactor vessels and stabilized over 2-wk using a culture medium. This stabilization period was followed by a 2-wk control period during which the microbiota was monitored. The microbiota was then subjected to a 4-wk treatment period by adding 5 mL of sterile peptone water with L. acidophilus CRL1014 at the concentration of 108 CFU-mL to vessel one the stomach compartment. Plate counts, Denaturing Gradient Gel Electrophoresis DGGE, short-chain fatty acid SCFA and ammonium analyses were carried out for monitoring of the microbial community from the colon compartments. Results: A significant increase p 



Autor: Sivieri, Katia; Morales, Martha L Villarreal; Adorno, Maria A Tallarico; Sakamoto, Isabel Kimiko; Saad, Susana M Isay; Rossi, Elizeu A

Fuente: https://archive.org/







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