Vol 62: Liver Kinase B1 Is Required for White Adipose Tissue Growth and Differentiation.Reportar como inadecuado



 Vol 62: Liver Kinase B1 Is Required for White Adipose Tissue Growth and Differentiation.


Vol 62: Liver Kinase B1 Is Required for White Adipose Tissue Growth and Differentiation. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Descargar gratis o leer online en formato PDF el libro: Vol 62: Liver Kinase B1 Is Required for White Adipose Tissue Growth and Differentiation.
This article is from Diabetes, volume 62.AbstractWhite adipose tissue WAT is not only a lipogenic and fat storage tissue but also an important endocrine organ that regulates energy homeostasis, lipid metabolism, appetite, fertility, and immune and stress responses. Liver kinase B1 LKB1, a tumor suppressor, is a key regulator in energy metabolism. However, the role of LKB1 in adipogenesis is unknown. The current study aimed to determine the contributions of LKB1 to adipogenesis in vivo. Using the Fabp4-Cre-loxP system, we generated adipose tissue–specific LKB1 knockout LKB1ad−-− mice. LKB1ad−-− mice exhibited a reduced amount of WAT, postnatal growth retardation, and early death before weaning. Further, LKB1 deletion markedly reduced the levels of insulin receptor substrate 1 IRS1, peroxisome proliferator–activated receptor γ, CCAAT-enhancer-binding protein α, and phosphorylated AMP-activated protein kinase AMPK. Consistent with these results, overexpression of constitutively active AMPK partially ablated IRS1 degradation in LKB1-deficient cells. LKB1 deletion increased the levels of F-box-WD repeat-containing protein Fbw 8, the IRS1 ubiquitination E3 ligase. Silencing of Fbw8 increased IRS1 levels. Finally, promoter analysis and DNA chromatin immunoprecipitation analysis identified three sterol regulatory element SRE sites in the Fbw8 promoter, where SRE-binding protein 1c binds and induces the expression of Fbw8. Taken together, these data indicate that LKB1 controls IRS1-dependent adipogenesis via AMPK in WAT.



Autor: Zhang, Wencheng; Wang, Qilong; Song, Ping; Zou, Ming-Hui

Fuente: https://archive.org/







Documentos relacionados