Vol 2: Lithium induces microcysts and polyuria in adolescent rat kidney independent of cyclooxygenase-2.Report as inadecuate



 Vol 2: Lithium induces microcysts and polyuria in adolescent rat kidney independent of cyclooxygenase-2.


Vol 2: Lithium induces microcysts and polyuria in adolescent rat kidney independent of cyclooxygenase-2. - Download this document for free, or read online. Document in PDF available to download.

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This article is from Physiological Reports, volume 2.AbstractIn patients, chronic treatment with lithium leads to renal microcysts and nephrogenic diabetes insipidus NDI. It was hypothesized that renal cyclooxygenase‐2 COX‐2 activity promotes microcyst formation and NDI. Kidney microcysts were induced in male adolescent rats by feeding dams with lithium 50 mmol-kg chow from postnatal days 7–34. Lithium treatment induced somatic growth retardation, renal microcysts and dilatations in cortical collecting duct; it increased cortical cell proliferation and inactive pGSK‐3β abundance; it lowered aquaporin‐2 AQP2 protein abundance and induced polyuria with decreased ability to concentrate the urine; and it increased COX‐2 protein level in thick ascending limb. Concomitant treatment with lithium and a specific COX‐2 inhibitor, parecoxib 5 mg-kg per day, P10–P34, did not prevent lithium‐induced microcysts and polyuria, but improved urine concentrating ability transiently after a 1‐desamino‐8‐D‐arginine vasopressin challenge. COX‐2 inhibition did not reduce cortical lithium‐induced cell proliferation and phosphorylation of glycogen synthase kinase‐3β GSK‐3β. COX‐1 protein abundance increased in rat kidney cortex in response to lithium. COX‐1 immunoreactivity was found in microcyst epithelium in rat kidney. A human nephrectomy specimen from a patient treated for 28 years with lithium displayed multiple, COX‐1‐immunopositive, microcysts. In chronic lithium‐treated adolescent rats, COX‐2 is not colocalized with microcystic epithelium, mitotic activity, and inactive pGSK‐3β in collecting duct; a blocker of COX‐2 does not prevent cell proliferation, cyst formation, or GSK‐3β inactivation. It is concluded that COX‐2 activity is not the primary cause for microcysts and polyuria in a NaCl‐substituted rat model of lithium nephropathy. COX‐1 is a relevant candidate to affect the injured epithelium.



Author: Kjaersgaard, Gitte; Madsen, Kirsten; Marcussen, Niels; Jensen, Boye L.

Source: https://archive.org/







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