Vol 8: Live Multicellular Tumor Spheroid Models For High-Content Imaging and Screening In Cancer Drug Discovery.Report as inadecuate



 Vol 8: Live Multicellular Tumor Spheroid Models For High-Content Imaging and Screening In Cancer Drug Discovery.


Vol 8: Live Multicellular Tumor Spheroid Models For High-Content Imaging and Screening In Cancer Drug Discovery. - Download this document for free, or read online. Document in PDF available to download.

Download or read this book online for free in PDF: Vol 8: Live Multicellular Tumor Spheroid Models For High-Content Imaging and Screening In Cancer Drug Discovery.
This article is from Current Chemical Genomics and Translational Medicine, volume 8.AbstractThe multi cellular tumor spheroid MCTS model has been used for decades with proven superiority over monolayer cell culture models at recapitulating in vivo tumor growth. Yet its use in high-throughput drug discovery has been limited, particularly with image based screening, due to practical and technical hurdles. Here we report a significant advance in utilizing live MCTS models for high-content image based drug discovery. Using a validated GFP reporter CK5Pro-GFP of luminal breast cancer stem cells CSC, we developed an algorithm to quantify changes in CK5Pro-GFP expression levels for individual Z-stack planes local or as maximal projections of the summed Z-stacks global of MCTS. From these image sets, we can quantify the cross-sectional area of GFP positive cells, the fluorescence intensity of the GFP positive cells, and the percent of spheroid cross-sectional area that expresses CK5Pro-GFP.We demonstrate that acquiring data in this manner can be done in real time and is statistically robust Z’=0.85 for use in primary high-content screening cancer drug discovery.



Author: Reid, Brian G.; Jerjian, Taleen; Patel, Purvi; Zhou, Qiong; Yoo, Byong Hoon; Kabos, Peter; Sartorius, Carol A.; LaBarbera, Daniel V.

Source: https://archive.org/







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