Vol 47: Liver cancer stem cells are selectively enriched by low-dose cisplatin.Reportar como inadecuado



 Vol 47: Liver cancer stem cells are selectively enriched by low-dose cisplatin.


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This article is from Brazilian Journal of Medical and Biological Research, volume 47.AbstractAccumulating evidence has indicated the importance of cancer stem cells in carcinogenesis. The goal of the present study was to determine the effect of low-dose cisplatin on enriched liver cancer stem cells LCSCs. Human hepatoblastoma HepG2 cells were treated with concentrations of cisplatin ranging from 1 to 5 μg-mL. Cell survival and proliferation were evaluated using a tetrazolium dye MTT assay. LCSCs were identified using specific markers, namely aldehyde dehydrogenase-1 ALDH1 and CD133. The percentage of ALDH1+ or CD133+ cells was examined by flow cytometric analysis. The expression of ALDH1 and-or CD133 in HepG2 cells was determined by immunocytochemical analysis. Low-dose cisplatin treatment significantly decreased cell survival in HepG2 cells after 24 or 72 h. However, the percentage of LCSCs in the surviving cells was greatly increased. The percentage of ALDH1+ or CD133+ cells was increased in a time- and dose-dependent manner after treatment with 1-4 μg-mL cisplatin, whereas 5 μg-mL cisplatin exposure slightly reduced the number of positive cells. These findings indicate that low-dose cisplatin treatment may efficiently enrich the LCSC population in HepG2 cells.



Autor: Zhang, H.; Chang, W.J.; Li, X.Y.; Zhang, N.; Kong, J.J.; Wang, Y.F.

Fuente: https://archive.org/







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