Vol 8: Induction of apoptosis by a hexane extract of aged black garlic in the human leukemic U937 cells.Reportar como inadecuado



 Vol 8: Induction of apoptosis by a hexane extract of aged black garlic in the human leukemic U937 cells.


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This article is from Nutrition Research and Practice, volume 8.AbstractBACKGROUND-OBJECTIVES: In this study, the apoptogenic activity and mechanisms of cell death induced by hexane extract of aged black garlic HEABG were investigated in human leukemic U937 cells. MATERIALS-METHODS: Cytotoxicity was evaluated by MTT 3-4, 5-dimethyl-thiazol-2-yl-2, 5-diphenyl tetrazoliumbromide assay. Apoptosis was detected using 4,6-diamidino-2-phenyllindile DAPI staining, agarose gel electrophoresis and flow cytometry. The protein levels were determined by Western blot analysis. Caspase activity was measured using a colorimetric assay. RESULTS: Exposure to HEABG was found to result in a concentration- and time-dependent growth inhibition by induction of apoptosis, which was associated with an up-regulation of death receptor 4 and Fas legend, and an increase in the ratio of Bax-Bcl-2 protein expression. Apoptosis-inducing concentrations of HEABG induced the activation of caspase-9, an initiator caspase of the mitochodrial mediated intrinsic pathway, and caspase-3, accompanied by proteolytic degradation of polyADP-ribose-polymerase. HEABG also induced apoptosis via a death receptor mediated extrinsic pathway by caspase-8 activation, resulting in the truncation of Bid, and suggesting the existence of cross-talk between the extrinsic and intrinsic pathways. However, pre-treatment of U937 cells with the caspase-3 inhibitor, z-DEVD-fmk, significantly blocked the HEABG-induced apoptosis of these cells, and increased the survival rate of HEABG-treated cells, confirming that HEABG-induced apoptosis is mediated through activation of caspase cascade. CONCLUSIONS: Based on the overall results, we suggest that HEABG reduces leukemic cell growth by inducing caspase-dependent apoptosis through both intrinsic and extrinsic pathways, implying its potential therapeutic value in the treatment of leukemia.



Autor: Park, Cheol; Park, Sejin; Chung, Yoon Ho; Kim, Gi-Young; Choi, Young Whan; Kim, Byung Woo; Choi, Yung Hyun

Fuente: https://archive.org/







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