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BMC Molecular Biology

, 11:79

First Online: 04 November 2010Received: 12 May 2010Accepted: 04 November 2010

Abstract

BackgroundCarcinoembryonic antigen cell adhesion molecule 1 CEACAM1 is a transmembrane protein with multiple functions in different cell types. CEACAM1 expression is frequently mis-regulated in cancer, with down-regulation reported in several tumors of epithelial origin and de novo expression of CEACAM1 in lung cancer and malignant melanoma. In this report we analyzed the regulation of CEACAM1 expression in three breast cancer cell lines that varied in CEACAM1 expression from none MCF7 to moderate MDA-MB-468 to high MCF10A, comparable to normal breast.

ResultsUsing in vivo footprinting and chromatin immunoprecipitation experiments we show that the CEACAM1 proximal promoter in breast cells is bound in its active state by SP1, USF1-USF2, and IRF1-2. When down-regulated the CEACAM1 promoter remains accessible to USF2 and partially accessible to USF1. Interferon-γ up-regulates CEACAM1 mRNA by a mechanism involving further induction of IRF-1 and USF1 binding at the promoter. As predicted by this analysis, silencing of IRF1 and USF1 but not USF2 by RNAi resulted in a significant decrease in CEACAM1 protein expression in MDA-MB-468 cells. The inactive CEACAM1 promoter in MCF7 cells exhibits decreased histone acetylation at the promoter region, with no evidence of H3K9 or H3K27 trimethylation, histone modifications often linked to condensed chromatin structure.

ConclusionsOur data suggest that transcription activators USF1 and IRF1 interact to modulate CEACAM1 expression and that the chromatin structure of the promoter is likely maintained in a poised state that can promote rapid induction under appropriate conditions.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2199-11-79 contains supplementary material, which is available to authorized users.

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Autor: Marieta Gencheva - Charng-Jui Chen - Tung Nguyen - John E Shively

Fuente: https://link.springer.com/



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