Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rateReportar como inadecuado




Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Microbial Cell Factories

, 9:93

First Online: 23 November 2010Received: 09 August 2010Accepted: 23 November 2010

Abstract

BackgroundYeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce.

ResultsHere, we report on cultivations under controlled specific growth rate of a GlycoSwitch-Man5 Pichia pastoris strain producing Granulocyte-Macrophage Colony-Stimulating Factor GM-CSF at high levels hundreds of milligrams per liter. We demonstrate that homogenous Man5GlcNAc2 N-glycosylation of the secreted proteins is achieved at all specific growth rates tested.

ConclusionsTogether, these data illustrate that the GlycoSwitch-Man5 P. pastoris is a robust production strain for homogenously N-glycosylated proteins.

List of AbbreviationsEFTelapsed fermentation time h

Fmethanol feed rate g-h

Smethanol concentration g-L

TCulture time h

Vbroth volume L

WCWwet cell weight

Xcell density g WCW-L

YX-SYield of biomass to substrate g-g

μSpecific growth rate under methanol conditions

μmaxmaximum specific growth rate under methanol conditions

νmaxmaximum specific methanol consumption rate g-g-h

Electronic supplementary materialThe online version of this article doi:10.1186-1475-2859-9-93 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Pieter P Jacobs - Mehmet Inan - Nele Festjens - Jurgen Haustraete - Annelies Van Hecke - Roland Contreras - Michael M Mea

Fuente: https://link.springer.com/







Documentos relacionados