Proteomic approach with LCMS-IT-TOF identified an increase of Rab33B after transient focal cerebral ischemia in miceReportar como inadecuado




Proteomic approach with LCMS-IT-TOF identified an increase of Rab33B after transient focal cerebral ischemia in mice - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Experimental and Translational Stroke Medicine

, 2:20

First Online: 23 November 2010Received: 10 June 2010Accepted: 23 November 2010

Abstract

BackgroundSeveral proteins are known to be markedly expressed in the brain during cerebral ischemia; however, the changes in protein profiles within the ischemic brain after an ischemic insult have not been fully elucidated. We studied the changes in the ischemic brain proteome after focal cerebral ischemia, induced by middle cerebral artery occlusion MCAO in mice.

MethodsLCMS-IT-TOF mass spectrometry was used to detect the changes in ischemic brain protein patterns after MCAO. We evaluated the protein expression detected in the ischemic area, by western blotting and immunohistochemistry.

ResultsNine unique proteins were identified from the ischemic area at 10 h after ischemic insult. Among these proteins, we focused on Rab33b, a member of RAS oncogene family and we found that Rab33b was up-regulated in the ischemic striatum and the number of Rab33B-positive cells increased in a time-dependent manner. Rab33B colocalized with Iba-1 positive microglia in the ischemic area.

ConclusionThese findings suggest that LCMS-IT-TOF is useful for identifying changes in proteins after cerebral ischemia and that Rab33B is partially related to the pathogenesis of transient cerebral ischemia in mice.

Electronic supplementary materialThe online version of this article doi:10.1186-2040-7378-2-20 contains supplementary material, which is available to authorized users.

Kana Hyakkoku, Junya Hamanaka contributed equally to this work.

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Autor: Kana Hyakkoku - Junya Hamanaka - Kazuhiro Tsuruma - Masamitsu Shimazawa - Hideaki Hara

Fuente: https://link.springer.com/



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