Protein adsorption on and swelling of polyelectrolyte brushes: A simultaneous ellipsometry-quartz crystal microbalance studyReportar como inadecuado




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Biointerphases

, Volume 5, Issue 4, pp 159–167

Received: 28 October 2010Accepted: 02 December 2010

Abstract

With a coupled spectroscopic ellipsometry-quartz crystal microbalance with dissipation QCM-D experimental setup, quantitative information can be obtained about the amount of buffer components water molecules and ions coupled to a polyacrylic acid PAA brush surface in swelling and protein adsorption processes. PAA Guiselin brushes with more than one anchoring point per single polymer chain were prepared. For the swollen brushes a high amount of buffer was found to be coupled to the brush-solution interface in addition to the content of buffer inside the brush layer. Upon adsorption of bovine serum albumin the further incorporation of buffer molecules into the protein-brush layer was monitored at overall electrostatic attractive conditions below the protein isolectric poimt IEP and electrostatic repulsive conditions above the protein IEP, and the shear viscosity of the combined polymer-protein layer was evaluated from QCM-D data. For adsorption at the -wrong side- of the IEP an incorporation of excess buffer molecules was observed, indicating an adjustment of charges in the combined polymer-protein layer. Desorption of protein at pH 7.6 led to a very high stretching of the polymer-protein layer with additional incorporation of high amounts of buffer, reflecting the increase of negative charges on the protein molecules at this elevated pH.

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Autor: Eva Bittrich - Keith Brian Rodenhausen - Klaus-Jochen Eichhorn - Tino Hofmann - Mathias Schubert - Manfred Stamm - Petra U

Fuente: https://link.springer.com/



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