Differential expression of microRNAs in GH-secreting pituitary adenomasReportar como inadecuado




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Diagnostic Pathology

, 5:79

First Online: 07 December 2010Received: 07 September 2010Accepted: 07 December 2010

Abstract

BackgroundThe purpose of this study was to 1 identify specific miRNAs in growth hormones GH-secreting pituitary adenomas; 2 determine the relationship between the expression of these miRNAs and tumor size, somatostatin analogs treatment, and responsiveness to somatostatin analogs SSA.

MethodsFifteen GH-secreting adenomas patients were treated with lanreotide for 4 months before surgery. Patients with 50% reduction of GH secretion by lanreotide were considered as SSA responders, while patients with less than 50% of GH reduction were considered as SSA nonresponders. We analyzed the miRNAs in 21 GH-secreting pituitary adenomas and 6 normal pituitaries by miRCURY™ LNA array and some differentially expressed miRNAs were validated by quantitative real-time PCR.

ResultsFifty-two miRNAs were differentially expressed between GH-secreting pituitary adenomas and normal pituitaries. Differential expression of 9 miRNAs was observed between micro- and macro-adenomas. Thirteen miRNAs were differentially expressed between tumor samples from lanreotide-treated patients and those from lanreotide-untreated patients. Seven miRNAs were differentially expressed between SSA responders or GH nonresponders. Several identified miRNAs may be involved in cell proliferation, apoptosis, cancer development and progression.

ConclusionsOur results indicate that altered miRNAs expression is involved in GH-secreting pituitary adenomas transformation, which will shed light on the mechanisms for the treatment of acromegaly by SSA. Identification and characterization of the targets of altered miRNAs genes may elucidate molecular mechanisms involved in the pathogenesis of pituitary adenoma.

Electronic supplementary materialThe online version of this article doi:10.1186-1746-1596-5-79 contains supplementary material, which is available to authorized users.

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Autor: Zhi-Gang Mao - Dong-Sheng He - Jing Zhou - Bin Yao - Wei-Wei Xiao - Chun-Hua Chen - Yong-Hong Zhu - Hai-Jun Wang

Fuente: https://link.springer.com/







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