Improved Efficacy of a Gene Optimised Adenovirus-based Vaccine for Venezuelan Equine Encephalitis VirusReportar como inadecuado




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Virology Journal

, 6:118

First Online: 31 July 2009Received: 30 March 2009Accepted: 31 July 2009

Abstract

BackgroundOptimisation of genes has been shown to be beneficial for expression of proteins in a range of applications. Optimisation has increased protein expression levels through improved codon usage of the genes and an increase in levels of messenger RNA. We have applied this to an adenovirus ad-based vaccine encoding structural proteins E3-E2-6K of Venezuelan equine encephalitis virus VEEV.

ResultsFollowing administration of this vaccine to Balb-c mice, an approximately ten-fold increase in antibody response was elicited and increased protective efficacy compared to an ad-based vaccine containing non-optimised genes was observed after challenge.

ConclusionThis study, in which the utility of optimising genes encoding the structural proteins of VEEV is demonstrated for the first time, informs us that including optimised genes in gene-based vaccines for VEEV is essential to obtain maximum immunogenicity and protective efficacy.

Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-6-118 contains supplementary material, which is available to authorized users.

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Autor: Amanda J Williams - Lyn M O-Brien - Robert J Phillpotts - Stuart D Perkins

Fuente: https://link.springer.com/



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