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Virology Journal

, 6:148

First Online: 25 September 2009Received: 25 June 2009Accepted: 25 September 2009

Abstract

BackgroundWe conducted molecular analyses to confirm four clustering HIV-1 infections Patient A, B, C and D in Guangzhou, China. These cases were identified by epidemiological investigation and suspected to acquire the infection through a common heterosexual transmission chain.

MethodsEnv C2V3V4 region, gag p17-p24 junction and partial pol gene of HIV-1 genome from serum specimens of these infected cases were amplified by reverse transcription polymerase chain reaction RT-PCR and nucleotide sequenced.

ResultsPhylogenetic analyses indicated that their viral nucleotide sequences were significantly clustered together bootstrap value is 99%, 98% and 100% in env, gag and pol tree respectively. Evolutionary distance analysis indicated that their genetic diversities of env, gag and pol genes were significantly lower than non-clustered controls, as measured by unpaired t-test env gene comparison: p < 0.005; gag gene comparison: p < 0.005; pol gene comparison: p < 0.005.

ConclusionEpidemiological results and molecular analyses consistently illustrated these four cases represented a transmission chain which dispersed in the locality through heterosexual contact involving commercial sex worker.

Electronic supplementary materialThe online version of this article doi:10.1186-1743-422X-6-148 contains supplementary material, which is available to authorized users.

Zhigang Han, Tommy WC Leung, Jinkou Zhao contributed equally to this work.

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Autor: Zhigang Han - Tommy WC Leung - Jinkou Zhao - Ming Wang - Lirui Fan - Kai Li - Xinli Pang - Zhenbo Liang - Wilina WL Lim

Fuente: https://link.springer.com/







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