Utility of a dual immunostain cocktail comprising of p53 and CK20 to aid in the diagnosis of non-neoplastic and neoplastic bladder biopsiesReportar como inadecuado




Utility of a dual immunostain cocktail comprising of p53 and CK20 to aid in the diagnosis of non-neoplastic and neoplastic bladder biopsies - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Diagnostic Pathology

, 4:35

First Online: 14 October 2009Received: 26 July 2009Accepted: 14 October 2009

Abstract

BackgroundDistinction between non-neoplastic and neoplastic bladder lesions is therapeutically and prognostically important. Our objective is to describe the use of double immunohistochemistry DIHC for p53+CK20 as a tool for diagnosing neoplasia in bladder biopsies.

Methodsp53+CK20 DIHC were examined in 38 reactive atypia, 10 dysplasia, 9 carcinoma in situ CIS and 7 invasive carcinoma IC cases. CK20 was evaluated according to distribution extent and degree of intensity whereas percentage of positive cells together with staining intensity was taken into account in the evaluation of p53.

Results92% of reactive cases were either CK20- or + only in the upper 1-3 urothelium. In dysplastic cases CK20 staining distribution was as follows: 60% in 2-3 of the urothelium, 30% full thickness, 10% in the upper 1-3 urothelium. Among CIS cases, 89% had full thickness CK20 positivity, of which 62% were p53+. 71% of IC cases exhibited strong and full thickness dual staining.

ConclusionThis is the first study in the literature to use DIHC of p53+CK20 in distinction of non-neoplastic and neoplastic bladder lesions. Dual staining by p53+CK20 cocktail allows for histologic correlation and diminishes the risk of losing the area of interest in limited biopsy specimens.

AbbreviationsDIHCDouble immunohistochemistry

CISCarcinoma in situ

ICinvasive carcinoma

WHO-ISUPWorld health organization-international society of urological pathology

RUAReactive urothelial atypia

AUSAtypia of unknown significance

UDUrothelial dysplasia

IHCImmunohistochemistry.

Electronic supplementary materialThe online version of this article doi:10.1186-1746-1596-4-35 contains supplementary material, which is available to authorized users.

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Autor: Isil Z Yildiz - Rosemary Recavarren - Henry B Armah - Sheldon Bastacky - Rajiv Dhir - Anil V Parwani

Fuente: https://link.springer.com/







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