Structural insights into the substrate tunnel of Saccharomyces cerevisiae carbonic anhydrase Nce103Reportar como inadecuado




Structural insights into the substrate tunnel of Saccharomyces cerevisiae carbonic anhydrase Nce103 - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Structural Biology

, 9:67

First Online: 24 October 2009Received: 19 December 2008Accepted: 24 October 2009

Abstract

BackgroundThe carbonic anhydrases CAs are involved in inorganic carbon utilization. They have been classified into six evolutionary and structural families: α-, β-, γ-, δ-, ε-, ζ- CAs, with β-CAs present in higher plants, algae and prokaryotes. The yeast Saccharomyces cerevisiae encodes a single copy of β-CA Nce103-YNL036W.

ResultsWe determined the crystal structure of Nce103 in complex with a substrate analog at 2.04 Å resolution. It assembles as a homodimer, with the active site located at the interface between two monomers. At the bottom of the substrate pocket, a zinc ion is coordinated by the three highly conserved residues Cys57, His112 and Cys115 in addition to a water molecule. Residues Asp59, Arg61, Gly111, Leu102, Val80, Phe75 and Phe97 form a tunnel to the bottom of the active site which is occupied by a molecule of the substrate analog acetate. Activity assays of full length and two truncated versions of Nce103 indicated that the N-terminal arm is indispensable.

ConclusionThe quaternary structure of Nce103 resembles the typical plant type β-CAs of known structure, with an N-terminal arm indispensable for the enzymatic activity. Comparative structure analysis enables us to draw a possible tunnel for the substrate to access the active site which is located at the bottom of a funnel-shaped substrate pocket.

Electronic supplementary materialThe online version of this article doi:10.1186-1472-6807-9-67 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Yan-Bin Teng - Yong-Liang Jiang - Yong-Xing He - Wei-Wei He - Fu-Ming Lian - Yuxing Chen - Cong-Zhao Zhou

Fuente: https://link.springer.com/



DESCARGAR PDF




Documentos relacionados