Preliminary molecular characterization of the human pathogen Angiostrongylus cantonensisReportar como inadecuado




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BMC Molecular Biology

, 10:97

First Online: 25 October 2009Received: 22 February 2009Accepted: 25 October 2009

Abstract

BackgroundHuman angiostrongyliasis is an emerging food-borne public health problem, with the number of cases increasing worldwide, especially in mainland China. Angiostrongylus cantonensis is the causative agent of this severe disease. However, little is known about the genetics and basic biology of A. cantonensis.

ResultsA cDNA library of A. cantonensis fourth-stage larvae was constructed, and ~1,200 clones were sequenced. Bioinformatic analyses revealed 378 cDNA clusters, 54.2% of which matched known genes at a cutoff expectation value of 10. Of these 378 unique cDNAs, 168 contained open reading frames encoding proteins containing an average of 238 amino acids. Characterization of the functions of these encoded proteins by Gene Ontology analysis showed enrichment in proteins with binding and catalytic activity. The observed pattern of enzymes involved in protein metabolism, lipid metabolism and glycolysis may reflect the central nervous system habitat of this pathogen. Four proteins were tested for their immunogenicity using enzyme-linked immunosorbent assays and histopathological examinations. The specificity of each of the four proteins was superior to that of crude somatic and excretory-secretory antigens of larvae, although their sensitivity was relatively low. We further showed that mice immunized with recombinant cystatin, a product of one of the four cDNA candidate genes, were partially protected from A. cantonensis infection.

ConclusionThe data presented here substantially expand the available genetic information about the human pathogen A. cantonensis, and should be a significant resource for angiostrongyliasis researchers. As such, this work serves as a starting point for molecular approaches for diagnosing and controlling human angiostrongyliasis.

AbbreviationsL3third-stage larva

L4fourth-stage larva

CNScentral nervous system

EMEeosinophilic meningoencephalitis

EoMeosinophilic meningitis

EoEeosinophilic encephalitis

ORFopen reading frame

UTRuntranslated region

BBBblood-brain barrier

ESexcretory-secretory

ELISAenzyme-linked immunosorbent assay

FRbpfatty acid and retinol-binding protein

GnRHRgonadotropin-releasing hormone receptor

IFsintermediate filaments

LDHlactic acid dehydrogenase. SD: standard deviations

PBSphosphate-buffered saline.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2199-10-97 contains supplementary material, which is available to authorized users.

Hualiang He, Mei Cheng contributed equally to this work.

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Autor: Hualiang He - Mei Cheng - Xiao Yang - Jinxiu Meng - Ai He - Xiaoying Zheng - Zhuoya Li - Pengjuan Guo - Zhihua Pan - Xime

Fuente: https://link.springer.com/



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