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BMC Infectious Diseases

, 9:177

First Online: 11 November 2009Received: 25 May 2009Accepted: 11 November 2009

Abstract

BackgroundWe have previously reported the use of PCR Melting Profile PCR MP technique based on using low denaturation temperatures during ligation mediated PCR LM PCR for bacterial strain differentiation. The aim of the current study was to evaluate this method for intra-species differentiation of Candida albicans strains.

MethodsIn total 123 Candida albicans strains including 7 reference, 11 clinical unrelated, and 105 isolates from patients of two hospitals in Poland were examined using three genotyping methods: PCR MP, macrorestriction analysis of the chromosomal DNA by pulsed-field gel electrophoresis REA-PFGE and RAPD techniques.

ResultsThe genotyping results of the PCR MP were compared with results from REA-PFGE and RAPD techniques giving 27, 26 and 25 unique types, respectively. The results showed that the PCR MP technique has at least the same discriminatory power as REA-PFGE and RAPD.

ConclusionData presented here show for the first time the evaluation of PCR MP technique for candidial strains differentiation and we propose that this can be used as a relatively simple and cheap technique for epidemiological studies in short period of time in hospital.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2334-9-177 contains supplementary material, which is available to authorized users.

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Autor: Beata Krawczyk - Justyna Leibner-Ciszak - Anna Mielech - Magdalena Nowak - Józef Kur

Fuente: https://link.springer.com/







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