Growth-differentiation factor-8 GDF-8 in the uterus: its identification and functional significance in the golden hamsterReportar como inadecuado




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Reproductive Biology and Endocrinology

, 7:134

First Online: 25 November 2009Received: 04 August 2009Accepted: 25 November 2009

Abstract

Transforming growth factor-beta superfamily regulates many aspects of reproduction in the female. We identified a novel member of this family, growth-differentiation factor 8 GDF-8 in the 72 h post coital uterine fluid of the golden hamster by proteomic techniques. Uterine GDF-8 mRNA decreased as pregnancy progressed while its active protein peaked at 72 h post coitus hpc and thereafter stayed at a lower level. At 72 hpc, the GDF-8 transcript was localized to the endometrial epithelium while its protein accumulated in the stroma. Exogenous GDF-8 slowed down proliferation of primary cultures of uterine smooth muscle cells SMC and endometrial epithelial cells EEC. In addition, GDF-8 attenuated the release of LIF leukaemia inhibiting factor by EEC. As for the embryo in culture, GDF-8 promoted proliferation of the trophotoderm TM and hatching but discouraged attachment. Our study suggests that GDF-8 could regulate the behavior of preimplantation embryos and fine-tune the physiology of uterine environment during pregnancy.

Electronic supplementary materialThe online version of this article doi:10.1186-1477-7827-7-134 contains supplementary material, which is available to authorized users.

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Autor: Chun Lung Wong - Ya Yu Huang - Wing Kei Ho - Hong Kit Poon - Pui Lai Cheung - Wai Sum O - Pak Ham Chow

Fuente: https://link.springer.com/







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