Tyrosine phosphorylation of the N-Methyl-D-Aspartate receptor 2B subunit in spinal cord contributes to remifentanil-induced postoperative hyperalgesia: the preventive effect of ketamineReportar como inadecuado




Tyrosine phosphorylation of the N-Methyl-D-Aspartate receptor 2B subunit in spinal cord contributes to remifentanil-induced postoperative hyperalgesia: the preventive effect of ketamine - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Molecular Pain

, 5:76

First Online: 30 December 2009Received: 30 October 2009Accepted: 30 December 2009

Abstract

BackgroundExperimental and clinical studies showed that intraoperative infusionof remifentanil has been associated with postoperative hyperalgesia. Previous reports suggested that spinal N-methyl-D-aspartate NMDA receptors may contribute to the development and maintenance of opioid-induced hyperalgesia. In the present study, we used a rat model of postoperative pain to investigate the role of tyrosine phosphorylation of NMDA receptor 2B NR2B subunit in spinal cord in the postoperative hyperalgesia induced by remifentanil and the intervention of pretreatment with ketamine.

ResultsIntraoperative infusion of remifentanil 0.04 mg-kg, subcutaneous significantly enhanced mechanical allodynia and thermal hyperalgesia induced by the plantar incision during the postoperative period each lasting between 2 h and 48 h, which was attenuated by pretreatment with ketamine 10 mg-kg, subcutaneous. Correlated with the pain behavior changes, immunocytochemical and western blotting experiments in our study revealed that there was a marked increase in NR2B phosphorylation at Tyr1472 in the superficial dorsal horn after intraoperative infusion of remifentanil, which was attenuated by pretreatment with ketamine.

ConclusionsThis study provides direct evidence that tyrosine phosphorylation of the NR2B at Tyr1472 in spinal dosal horn contributes to postoperative hyperalgesia induced by remifentanil and supports the potential therapeutic value of ketamine for improving postoperative hyperalgesia induced by remifentanil.

List of abbreviations usedNMDAN-methyl-D-aspartate

NR2BN-Methyl-D-Aspartate receptor 2B subunit

Py-NR2Btyrosine phosphorylation of NR2B

Tyr-1472tyrosine 1472

LTPlong-term potentiation.

Electronic supplementary materialThe online version of this article doi:10.1186-1744-8069-5-76 contains supplementary material, which is available to authorized users.

Xiaoping Gu, Xiaoli Wu contributed equally to this work.

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Autor: Xiaoping Gu - Xiaoli Wu - Yue Liu - Songqin Cui - Zhengliang Ma

Fuente: https://link.springer.com/







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